BRENDA - Enzyme Database

An alpha-L-fucosidase from Penicillium multicolor as a candidate enzyme for the synthesis of alpha (1-3)-linked fucosyl oligosaccharides by transglycosylation

Ajisaka, K.; Fujimoto, H.; Miyasato, M.; Carbohydr. Res. 309, 125-129 (1998)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
extracellular
-
Aspergillus niger
-
-
3.2.1.51
extracellular
-
Penicillium multicolor
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
180000
-
gel filtration
Penicillium multicolor
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
Penicillium multicolor
same yield of transglycosylation and hydrolysis products
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
Penicillium multicolor
?
3.2.1.51
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
Penicillium multicolor
slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
Penicillium multicolor
?
3.2.1.51
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
Penicillium multicolor
same yield of transglycosylation and hydrolysis products
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
-
Penicillium multicolor
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc + H2O
Aspergillus niger
slow hydrolysis rate
alpha-L-fucose + N-acetyl-D-glucosamine
-
Aspergillus niger
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc + H2O
Penicillium multicolor
slow hydrolysis rate
alpha-L-fucose + N-acetyl-D-glucosamine
-
Penicillium multicolor
?
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.2.1.51
dimethyl formamide
-
Aspergillus niger
3.2.1.51
dimethyl formamide
more stable than the Aspergillus niger enzyme, no loss of activity up to 10%, v/v
Penicillium multicolor
3.2.1.51
dimethyl sulfoxide
-
Aspergillus niger
3.2.1.51
dimethyl sulfoxide
more stable than the Aspergillus niger enzyme, no loss of activity up to 20%, v/v
Penicillium multicolor
3.2.1.51
dioxane
-
Aspergillus niger
3.2.1.51
dioxane
more stable than the Aspergillus niger enzyme, no loss of activity up to 10%, v/v
Penicillium multicolor
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Aspergillus niger
-
-
-
3.2.1.51
Penicillium multicolor
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
partial
Penicillium multicolor
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
commercial preparation
lactase-P
Penicillium multicolor
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
0.04
-
-
Penicillium multicolor
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
transglycosylation activity
208697
Aspergillus niger
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
no other isomer
208697
Aspergillus niger
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
transglycosylation activity
208697
Penicillium multicolor
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
no other isomer
208697
Penicillium multicolor
?
3.2.1.51
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
same yield of transglycosylation and hydrolysis products
208697
Penicillium multicolor
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
208697
Penicillium multicolor
?
3.2.1.51
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
208697
Penicillium multicolor
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
208697
Penicillium multicolor
?
3.2.1.51
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
same yield of transglycosylation and hydrolysis products
208697
Penicillium multicolor
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
-
208697
Penicillium multicolor
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc + H2O
slow hydrolysis rate
208697
Aspergillus niger
alpha-L-fucose + N-acetyl-D-glucosamine
-
208697
Aspergillus niger
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc + H2O
slow hydrolysis rate
208697
Penicillium multicolor
alpha-L-fucose + N-acetyl-D-glucosamine
-
208697
Penicillium multicolor
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
50
-
stable up to
Aspergillus niger
3.2.1.51
50
-
stable up to
Penicillium multicolor
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5
-
-
Penicillium multicolor
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
3
7
stable within
Penicillium multicolor
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
extracellular
-
Aspergillus niger
-
-
3.2.1.51
extracellular
-
Penicillium multicolor
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
180000
-
gel filtration
Penicillium multicolor
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
Penicillium multicolor
same yield of transglycosylation and hydrolysis products
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
Penicillium multicolor
?
3.2.1.51
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
Penicillium multicolor
slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
Penicillium multicolor
?
3.2.1.51
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
Penicillium multicolor
same yield of transglycosylation and hydrolysis products
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
-
Penicillium multicolor
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc + H2O
Aspergillus niger
slow hydrolysis rate
alpha-L-fucose + N-acetyl-D-glucosamine
-
Aspergillus niger
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc + H2O
Penicillium multicolor
slow hydrolysis rate
alpha-L-fucose + N-acetyl-D-glucosamine
-
Penicillium multicolor
?
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.2.1.51
dimethyl formamide
-
Aspergillus niger
3.2.1.51
dimethyl formamide
more stable than the Aspergillus niger enzyme, no loss of activity up to 10%, v/v
Penicillium multicolor
3.2.1.51
dimethyl sulfoxide
-
Aspergillus niger
3.2.1.51
dimethyl sulfoxide
more stable than the Aspergillus niger enzyme, no loss of activity up to 20%, v/v
Penicillium multicolor
3.2.1.51
dioxane
-
Aspergillus niger
3.2.1.51
dioxane
more stable than the Aspergillus niger enzyme, no loss of activity up to 10%, v/v
Penicillium multicolor
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
partial
Penicillium multicolor
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
commercial preparation
lactase-P
Penicillium multicolor
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
0.04
-
-
Penicillium multicolor
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
transglycosylation activity
208697
Aspergillus niger
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
no other isomer
208697
Aspergillus niger
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
transglycosylation activity
208697
Penicillium multicolor
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
no other isomer
208697
Penicillium multicolor
?
3.2.1.51
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
same yield of transglycosylation and hydrolysis products
208697
Penicillium multicolor
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
208697
Penicillium multicolor
?
3.2.1.51
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
208697
Penicillium multicolor
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
208697
Penicillium multicolor
?
3.2.1.51
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
same yield of transglycosylation and hydrolysis products
208697
Penicillium multicolor
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
-
208697
Penicillium multicolor
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc + H2O
slow hydrolysis rate
208697
Aspergillus niger
alpha-L-fucose + N-acetyl-D-glucosamine
-
208697
Aspergillus niger
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc + H2O
slow hydrolysis rate
208697
Penicillium multicolor
alpha-L-fucose + N-acetyl-D-glucosamine
-
208697
Penicillium multicolor
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
50
-
stable up to
Aspergillus niger
3.2.1.51
50
-
stable up to
Penicillium multicolor
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5
-
-
Penicillium multicolor
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
3
7
stable within
Penicillium multicolor