BRENDA - Enzyme Database

Alteration of the isoform composition of plasma-membrane-associated rat sperm alpha-L-fucosidase during late epididymal maturation: Comparative characterization of the acidic and neutral isoforms

Abascal, I.; Skalaban, S.R.; Grimm, K.M.; Aviles, M.; Martinez-Menarguez, J.A.; Biochem. J. 333, 201-207 (1998)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.4
-
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Rattus sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
plasma membrane
-
Rattus sp.
5886
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Rattus sp.
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
isoform A derived by sialylation of isoform B near the end of epididymal maturation
Rattus sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
semen
two isoforms: A, B, characterized during epididymal maturation
Rattus sp.
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208696
Rattus sp.
4-methylumbelliferone + alpha-L-fucose
-
208696
Rattus sp.
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
50
-
isoform A: 50% inactivation in 1 h, isoform B: 50% inactivation in 10 min
Rattus sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
7
-
-
Rattus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.4
-
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Rattus sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
plasma membrane
-
Rattus sp.
5886
-
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
isoform A derived by sialylation of isoform B near the end of epididymal maturation
Rattus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
semen
two isoforms: A, B, characterized during epididymal maturation
Rattus sp.
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208696
Rattus sp.
4-methylumbelliferone + alpha-L-fucose
-
208696
Rattus sp.
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
50
-
isoform A: 50% inactivation in 1 h, isoform B: 50% inactivation in 10 min
Rattus sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
7
-
-
Rattus sp.