BRENDA - Enzyme Database

Purification of Pomacea canaliculata ?-Fucosidase Isoforms with Different Thermostabilities.

Hirata, K.; Aso, Y.; Yasuda, S.; Ishiguro, M.; Biosci. Biotechnol. Biochem. 62, 807-810 (1998)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.23
-
4-nitrophenyl-alpha-L-fucopyranoside
isoform F2
Pomacea canaliculata
3.2.1.51
0.242
-
4-nitrophenyl-alpha-L-fucopyranoside
isoform F1
Pomacea canaliculata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
260000
-
gel filtration
Pomacea canaliculata
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Pomacea canaliculata
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
4.7%, w/w, mannose content for isoform F1, 5%, w/w, mannose content for isoforms F2
Pomacea canaliculata
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, chromatography techniques, two isoforms: F1, F2
Pomacea canaliculata
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
viscus
-
Pomacea canaliculata
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.51
5.69
-
isoform F2
Pomacea canaliculata
3.2.1.51
5.89
-
isoform F1
Pomacea canaliculata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208695
Pomacea canaliculata
4-nitrophenol + alpha-L-fucose
-
208695
Pomacea canaliculata
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
oligomer
contains subunits of 55 kDa and 52 kDa, SDS-PAGE
Pomacea canaliculata
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.2.1.51
60
65
isoform F2: stable up to
Pomacea canaliculata
3.2.1.51
65
-
isoform F1: stable up to
Pomacea canaliculata
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
3.5
5.5
isoforms F1
Pomacea canaliculata
3.2.1.51
4
6
isoforms F2
Pomacea canaliculata
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
4
7
isoform F1: stable within
Pomacea canaliculata
3.2.1.51
4.5
7
isoform F2: stable within
Pomacea canaliculata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.23
-
4-nitrophenyl-alpha-L-fucopyranoside
isoform F2
Pomacea canaliculata
3.2.1.51
0.242
-
4-nitrophenyl-alpha-L-fucopyranoside
isoform F1
Pomacea canaliculata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
260000
-
gel filtration
Pomacea canaliculata
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
4.7%, w/w, mannose content for isoform F1, 5%, w/w, mannose content for isoforms F2
Pomacea canaliculata
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, chromatography techniques, two isoforms: F1, F2
Pomacea canaliculata
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
viscus
-
Pomacea canaliculata
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.51
5.69
-
isoform F2
Pomacea canaliculata
3.2.1.51
5.89
-
isoform F1
Pomacea canaliculata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208695
Pomacea canaliculata
4-nitrophenol + alpha-L-fucose
-
208695
Pomacea canaliculata
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
oligomer
contains subunits of 55 kDa and 52 kDa, SDS-PAGE
Pomacea canaliculata
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.2.1.51
60
65
isoform F2: stable up to
Pomacea canaliculata
3.2.1.51
65
-
isoform F1: stable up to
Pomacea canaliculata
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
3.5
5.5
isoforms F1
Pomacea canaliculata
3.2.1.51
4
6
isoforms F2
Pomacea canaliculata
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
4
7
isoform F1: stable within
Pomacea canaliculata
3.2.1.51
4.5
7
isoform F2: stable within
Pomacea canaliculata