BRENDA - Enzyme Database

Recombinant canine alpha-L-fucosidase: Expression, purification, and characterization

Bielicki, J.; Muller, V.; Fuller, M.; Hopwood, J.J.; Anson, D.S.; Mol. Genet. Metab. 69, 24-32 (2000)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.2.1.51
medicine
recombinant enzyme will extend the proven efficiacy of enzyme replacement therapy in lysosomal storage disorders e.g. fucosidosis
Canis lupus lupus
Cloned(Commentary)
EC Number
Commentary
Organism
3.2.1.51
expression in CHO cells; expression in MDCK cells
Canis lupus lupus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.14
-
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Canis lupus lupus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
50000
-
2 * 50000, SDS-PAGE
Canis lupus lupus
3.2.1.51
156000
-
gel filtration
Canis lupus lupus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Canis lupus lupus
-
canine
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
10%, w/w, carbohydrate content
Canis lupus lupus
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, recombinant enzyme
Canis lupus lupus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
14.9
17.1
recombinant enzyme, value depending on expression systems and buffer
Canis lupus lupus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208694
Canis lupus lupus
4-methylumbelliferone + alpha-L-fucose
-
208694
Canis lupus lupus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
dimer
2 * 50000, SDS-PAGE
Canis lupus lupus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
7
-
fluorogenic substrate, natural substrates, isolated from dog brain, more readily digested at acidic pH
Canis lupus lupus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.51
5
9
-
Canis lupus lupus
Application (protein specific)
EC Number
Application
Commentary
Organism
3.2.1.51
medicine
recombinant enzyme will extend the proven efficiacy of enzyme replacement therapy in lysosomal storage disorders e.g. fucosidosis
Canis lupus lupus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
expression in CHO cells; expression in MDCK cells
Canis lupus lupus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.14
-
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Canis lupus lupus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
50000
-
2 * 50000, SDS-PAGE
Canis lupus lupus
3.2.1.51
156000
-
gel filtration
Canis lupus lupus
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
10%, w/w, carbohydrate content
Canis lupus lupus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, recombinant enzyme
Canis lupus lupus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
14.9
17.1
recombinant enzyme, value depending on expression systems and buffer
Canis lupus lupus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208694
Canis lupus lupus
4-methylumbelliferone + alpha-L-fucose
-
208694
Canis lupus lupus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
dimer
2 * 50000, SDS-PAGE
Canis lupus lupus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
7
-
fluorogenic substrate, natural substrates, isolated from dog brain, more readily digested at acidic pH
Canis lupus lupus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.51
5
9
-
Canis lupus lupus