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Literature summary extracted from

  • Sasaki, T.; Sukegawa, K.; Masue, M.; Fukuda, S.; Tomatsu, S.; Orii, T.
    Purification and partial characterization of alpha-N-acetylglucosaminidase from human liver (1991), J. Biochem., 110, 842-846.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.50 mouse anti-serum raised in BALB/c mice, complete inhibition at pH 7.5 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.50 0.13 0.2 p-nitrophenyl-alpha-N-acetylglucosaminide
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.50 80000
-
 SDS-PAGE Homo sapiens
3.2.1.50 300000 307000 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.50 (GlcNAcalpha(1-4))n + H2O Homo sapiens involved in the degradation of heparin and heparan sulfate (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.50 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.50
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.50 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.50 (GlcNAcalpha(1-4))n + H2O involved in the degradation of heparin and heparan sulfate Homo sapiens (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
 ?
3.2.1.50 p-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
 Homo sapiens p-nitrophenol + N-acetyl-alpha-D-glucosamine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.50 50 70 stable at 50°C for 1 h, at 55°C loss of 17%, at 60°C loss of 77% of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.50 4.5
-
 p-nitrophenyl-alpha-N-acetylglucosaminide Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.50 6.5 8.5
-
 Homo sapiens