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Literature summary extracted from

  • Mersman, G.; von Figura, K.; Buddecke, E.
    Physical properties and biological activities of two forms of alpha-N-acetylglucosaminidase from bovine spleen (1974), Biochim. Biophys. Acta, 364, 88-96.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.50 entire inactivation by gel electrophoresis in the presence of sodium dodecylsulfate Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.50 0.043 0.61 2-nitrophenyl alpha-N-acetylglucosaminide form I 0.067, form II 0.043 Bos taurus
3.2.1.50 0.12 1.6 phenyl-alpha-N-acetylglucosaminide form I 0.32, form II 0.29 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.50 Hg2+ complete inactivation at 20 mM HgCl2 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.50 64500
-
form II after gel filtration or disc electrophoresis Bos taurus
3.2.1.50 127500
-
form I after gel filtration or disc electrophoresis Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.50 (GlcNAcalpha(1-4))n + H2O Bos taurus involved in the degradation of heparin and heparan sulfate (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.50 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.50
-
Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.50 spleen
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.50 0.0075
-
purified enzyme Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.2.1.50 37°C, 4 days, no loss of activity, but complete dissociation of form I into form II Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.50 (GlcNAcalpha(1-4))n + H2O involved in the degradation of heparin and heparan sulfate Bos taurus (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
?
3.2.1.50 2-nitrophenyl alpha-N-acetylglucosaminide + H2O
-
Bos taurus 2-nitrophenol + alpha-N-acetylglucosamine
-
?
3.2.1.50 phenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
Bos taurus phenol + N-acetyl-alpha-D-glucosamine
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.50 2-acetamido-2-deoxy-alpha-D-glucoside acetamidodeoxyglucohydrolase
-
Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.50 50 70 in citrate buffer, pH 6.0, no inactivation by 50°C heating, 90% inactivation at 70°C for 30 min Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.50 4.7
-
phenyl-alpha-N-acetylglucosaminide Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.50 5 10.5 rapid loss of activity above and below Bos taurus