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Literature summary extracted from

  • Hultberg, B.; Lindsten, J.; Sjöblad, S.
    Molecular forms and activities of glycosidases in cultures of amniotic-fluid cells (1976), Biochem. J., 155, 599-605.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.50 medicine antenatal diagnosis of heritable lysosomal storage diseases Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.50 0.12 1.6 phenyl-alpha-N-acetylglucosaminide 1 M citrate buffer, pH 4.5 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.50 (GlcNAcalpha(1-4))n + H2O Homo sapiens
-
 (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.50 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.50 amnion
-
 Homo sapiens
-
3.2.1.50 amniotic fluid
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.50 0.00012
-
 phenyl-N-acetyl-alpha-D-glucosaminide as substrate Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.2.1.50 -20°C, 0.9% NaCl, cultivated cells centrifuged at 1500 * g, 2 weeks, no loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.50 (GlcNAcalpha(1-4))n + H2O
-
 Homo sapiens (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
 ?
3.2.1.50 phenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
 Homo sapiens phenol + N-acetyl-alpha-D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.50 2-acetamido-2-deoxy-alpha-D-glucoside acetamidodeoxyglucohydrolase
-
 Homo sapiens
3.2.1.50 N-acetyl-alpha-glucosaminidase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.50 4.5
-
 phenyl-alpha-N-acetylglucosaminide Homo sapiens