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Literature summary extracted from

  • Yoshigi, N.; Okada, Y.; Sahara, H.; Koshino, S.
    Expression in Escherichia coli of cDNA encoding barley beta-amylase and properties of recombinant beta-amylase (1994), Biosci. Biotechnol. Biochem., 58, 1080-1086.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.2 expression in Escherichia coli, the recombinant enzyme lacks 4 amino acids at positions 2-5 (Glu-Val-Asn-Val) when compared with barley beta-amylase Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.2 Ag+ 1 mM, almost complete inhibition of recombinant enzyme Hordeum vulgare
3.2.1.2 Cd2+ 1 mM, almost complete inhibition of recombinant enzyme Hordeum vulgare
3.2.1.2 Cu2+ 1 mM, almost complete inhibition of recombinant enzyme Hordeum vulgare
3.2.1.2 Hg2+ 1 mM, almost complete inhibition of recombinant enzyme Hordeum vulgare
3.2.1.2 PCMB 0.1 mM, complete inhibition of recombinant enzyme Hordeum vulgare
3.2.1.2 Zn2+ 1 mM, almost complete inhibition of recombinant enzyme Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.2 additional information
-
 additional information
-
 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.2 56000
-
 x * 56000, recombinant enzyme, SDS-PAGE Hordeum vulgare
3.2.1.2 59169
-
 x * 59169, recombinant enzyme, calculation from amino acid sequence Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.2 Hordeum vulgare
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.2 no glycoprotein contains no carbohydrate Hordeum vulgare

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.2 recombinant enzyme Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.2 amylopectin + H2O
-
 Hordeum vulgare beta-maltose + ?
-
 ?
3.2.1.2 amylose + H2O DP = 17 Hordeum vulgare beta-maltose
-
 ?
3.2.1.2 maltoheptaose + H2O
-
 Hordeum vulgare ?
-
 ?
3.2.1.2 maltohexaose + H2O
-
 Hordeum vulgare ?
-
 ?
3.2.1.2 maltopentaose + H2O
-
 Hordeum vulgare ?
-
 ?
3.2.1.2 maltotetraose + H2O
-
 Hordeum vulgare ?
-
 ?
3.2.1.2 starch + H2O soluble Hordeum vulgare maltose + ? beta-maltose ?

Subunits

EC Number Subunits Comment Organism
3.2.1.2 ? x * 59169, recombinant enzyme, calculation from amino acid sequence Hordeum vulgare
3.2.1.2 ? x * 56000, recombinant enzyme, SDS-PAGE Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.2 55
-
-
 Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.2 53
-
 stable up to Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.2 3.5 7.5
-
 Hordeum vulgare

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.2 3.5 9.5 stable Hordeum vulgare