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Literature summary extracted from

  • Serafimova, F.; Franz, A.; Werries, E.
    Detection, purification and partial characterization of beta-amylase from trophozoites of Entamoeba invadens (1996), Mol. Biochem. Parasitol., 83, 175-181.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.2 Hg2+
-
 Entamoeba invadens
3.2.1.2 PCMB
-
 Entamoeba invadens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.2 2-mercaptoethanol activates Entamoeba invadens
3.2.1.2 cysteine activates Entamoeba invadens
3.2.1.2 dithiothreitol activates Entamoeba invadens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.2 45000
-
 gel filtration Entamoeba invadens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.2 Entamoeba invadens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.2
-
 Entamoeba invadens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.2 trophozoite
-
 Entamoeba invadens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.2 65.1
-
-
 Entamoeba invadens

Storage Stability

EC Number Storage Stability Organism
3.2.1.2 4°C, stable for 3 weeks Entamoeba invadens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.2 4-nitrophenyl-maltoheptaoside + H2O
-
 Entamoeba invadens ?
-
 ?
3.2.1.2 amylopectin + H2O
-
 Entamoeba invadens beta-maltose + ? and limit dextrin ?
3.2.1.2 amylose + H2O
-
 Entamoeba invadens beta-maltose
-
 ?
3.2.1.2 glycogen + H2O
-
 Entamoeba invadens beta-maltose and limit dextrin ?
3.2.1.2 additional information no activity with pullulan Entamoeba invadens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.2 monomer 1 * 45000, SDS-PAGE Entamoeba invadens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.2 42
-
 degradation of glycogen Entamoeba invadens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.2 29 41 75% of maximal activity at 29°C and at 41°C Entamoeba invadens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.2 5.5
-
 degradation of glycogen Entamoeba invadens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.2 3 7.2 50% of maximal activity at pH 3.0 and pH 7.2, degradation of glycogen Entamoeba invadens