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Literature summary extracted from

  • Shen, G.J.; Saha, B.C.; Lee, Y.E.; Bhatnagar, L.; Zeikus, J.G.
    Purification and characterization of a novel thermostable beta-amylase from Clostridium thermosulphurogenes (1988), Biochem. J., 254, 835-840.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.2 alpha-cyclodextrin
-
 Thermoanaerobacterium thermosulfurigenes
3.2.1.2 beta-cyclodextrin
-
 Thermoanaerobacterium thermosulfurigenes
3.2.1.2 PCMB
-
 Thermoanaerobacterium thermosulfurigenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.2 additional information
-
 additional information Km: 2.29 mg/ml for soluble starch at 60°C, 1.68 mg/ml for soluble starch at 75°C Thermoanaerobacterium thermosulfurigenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.2 extracellular
-
 Thermoanaerobacterium thermosulfurigenes
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.2 51000
-
 4 * 51000, SDS-PAGE Thermoanaerobacterium thermosulfurigenes
3.2.1.2 210000
-
 gel filtration Thermoanaerobacterium thermosulfurigenes

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.2 Thermoanaerobacterium thermosulfurigenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.2
-
 Thermoanaerobacterium thermosulfurigenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.2 4215
-
-
 Thermoanaerobacterium thermosulfurigenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.2 amylopectin + H2O
-
 Thermoanaerobacterium thermosulfurigenes beta-maltose + ?
-
 ?
3.2.1.2 amylose + H2O
-
 Thermoanaerobacterium thermosulfurigenes beta-maltose
-
 ?
3.2.1.2 glycogen + H2O
-
 Thermoanaerobacterium thermosulfurigenes beta-maltose
-
 ?
3.2.1.2 additional information no activity with pullulan Thermoanaerobacterium thermosulfurigenes ?
-
 ?
3.2.1.2 starch + H2O soluble Thermoanaerobacterium thermosulfurigenes maltose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.2 tetramer 4 * 51000, SDS-PAGE Thermoanaerobacterium thermosulfurigenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.2 75
-
-
 Thermoanaerobacterium thermosulfurigenes

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.2 50 80 50°C: about 55% of maximal activity, 80°C: about 65% of maximal activity Thermoanaerobacterium thermosulfurigenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.2 additional information
-
 stability is greatly enhanced by addition of 5 mM CaCl2 Thermoanaerobacterium thermosulfurigenes
3.2.1.2 70
-
 2 h, stable in absence of substrate or Ca2+ Thermoanaerobacterium thermosulfurigenes
3.2.1.2 80
-
 unstable in absence of substrate or Ca2+, stable in presence of 5 mM Ca2+ or 1% substrate Thermoanaerobacterium thermosulfurigenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.2 5.5
-
-
 Thermoanaerobacterium thermosulfurigenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.2 2 8 pH 2.0: about 35% of maximal activity, pH 8.0: about 30% of maximal activity Thermoanaerobacterium thermosulfurigenes
3.2.1.2 5 6 more than 80% of maximal activity at pH 5.0 and at pH 6.0 Thermoanaerobacterium thermosulfurigenes

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.2 3.5 7 70°C, in presence of 5 mM CaCl2 Thermoanaerobacterium thermosulfurigenes