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Literature summary extracted from

  • Henderson, H.M.; McEwen, T.J.
    Effect of ascorbic acid on thioglucosidases from different crucifers (1972), Phytochemistry, 11, 3127-3133.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.147 ascorbic acid
-
 Brassica napus
3.2.1.147 ascorbic acid considerable activation in the pH range 5.7-7.5, maximal activation at 0.375 mM Sinapis alba

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Brassica napus
-
 cv. Turret
-
3.2.1.147 Brassica napus
-
 cv. Bronowski
-
3.2.1.147 Brassica rapa
-
 cv. Echo
-
3.2.1.147 Sinapis alba
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 sinigrin + H2O
-
 Brassica napus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Brassica rapa D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Sinapis alba D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.147 6 7.2 activity in presence of ascorbic acid is greater in sodium phosphate buffer than in citric acid /Na2HPO4 buffer Brassica napus