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Literature summary extracted from

  • Petroski, R.J.
    Stereoselectivity of the interaction of thioglucoside glucohydrolase and epithiospecifier protein from various sources (1986), Plant Sci., 44, 85-88.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Aspergillus sydowii
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Brassica rapa
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Sinapis alba
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Crambe hispanica subsp. abyssinica
3.2.1.147 epithiospecifier protein a mixture of products which includes 1-cyano-2-hydroxy-3-butene, (R)-5-vinyloxazolidine-2-thione, D-glucose, HSO4- and elemental sulfur is formed from (S)-2-hydroxy-3-butenylglucosinolate without epithiospecifier protein at pH 5.9. These products as well as erythro- and threo-1-cyano-2-hydroxy-3,4-epithiobutanes are formed by combination of the enzyme and epithiospecifier protein from various sources Sinapis alba

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Aspergillus sydowii
-
-
-
3.2.1.147 Brassica rapa
-
 ssp. rapifera
-
3.2.1.147 Crambe hispanica subsp. abyssinica
-
-
-
3.2.1.147 Sinapis alba
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 2-hydroxybut-3-enylglucosinolate + H2O (S)-2-hydroxy-3-butenylglucosinolate, i.e. epi-progoitrin Sinapis alba additional information a mixture of products which includes 1-cyano-2-hydroxy-3-butene, (R)-5-vinyloxazolidine-2-thione, D-glucose, HSO4- and elemental sulfur is formed without epithiospecifier protein at pH 5.9. These products as well as erythro-1-cyano-2-hydroxy-3,4-epithiobutanes and threo-1-cyano-2-hydroxy-3,4-epithiobutanes are formed by combination of the enzyme and epithiospecifier protein from various sources ?
3.2.1.147 sinigrin + H2O
-
 Aspergillus sydowii D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Brassica rapa D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Sinapis alba D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Crambe hispanica subsp. abyssinica D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?