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Literature summary extracted from

  • Hatakeyama, K.; Nemoto, Y.; Ueda, K.; Hayaishi, O.
    Purification and characterization of poly(ADP-ribose) glycohydrolase. Different modes of action on large and small poly(ADP-ribose) (1986), J. Biol. Chem., 261, 14902-14911.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.143 adenosine 5'-tetraphosphate 5 mM, slight activation Bos taurus
3.2.1.143 ADP 5 mM, slight activation Bos taurus
3.2.1.143 ADP-glucose 5 mM, slight activation Bos taurus
3.2.1.143 AMP 5 mM, slight activation Bos taurus
3.2.1.143 ATP 5 mM, slight activation Bos taurus
3.2.1.143 NADH 5 mM, slight activation Bos taurus
3.2.1.143 NADP+ 5 mM, slight activation Bos taurus
3.2.1.143 NADPH 5 mM, slight activation Bos taurus
3.2.1.143 P1,P5-di(adenosine-5')pentaphosphate 5 mM, slight activation Bos taurus

General Stability

EC Number General Stability Organism
3.2.1.143 poly(ADP-ribose) protects the enzyme from thermal inactivation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.143 2':3'-cyclic NADP+
-
 Bos taurus
3.2.1.143 acetone 20%, 95% inhibition Bos taurus
3.2.1.143 adenosine 3':5'-cyclic monophosphate
-
 Bos taurus
3.2.1.143 ADP-ribose 5 mM, 55% inhibition Bos taurus
3.2.1.143 DNA denatured; no inhibition by native DNA Bos taurus
3.2.1.143 ethanol 20%, 91% inhibition Bos taurus
3.2.1.143 histone histone H1, H2A, H2B, H3 and H4 Bos taurus
3.2.1.143 Poly(A)
-
 Bos taurus
3.2.1.143 RNA calf liver RNA Bos taurus
3.2.1.143 SDS 0.01%, 96% inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.143 0.0003
-
 Poly(ADP-ribose)
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.143 nucleus
-
 Bos taurus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.143 KCl 100 mM, slight activation Bos taurus
3.2.1.143 NaCl 100 mM, slight activation Bos taurus
3.2.1.143 Sodium phosphate 100 mM, slight activation Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.143 poly(ADP-ribose)n + H2O Bos taurus regulates differentially the levels of large and small poly(ADP-ribose) in the cell ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.143 Bos taurus
-
 calf
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.143 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.143 85
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.2.1.143 -20°C, retains 90-100% of its activity for at least 1.5 years Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.143 poly(ADP-ribose)n + H2O n greater 20, degradation proceeds in a biphasic as well as bimodal manner Bos taurus ADP-ribose
-
 ?
3.2.1.143 poly(ADP-ribose)n + H2O regulates differentially the levels of large and small poly(ADP-ribose) in the cell Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.143 50
-
-
 Bos taurus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.143 30 50 30°C: about 25% of maximal activity, 55°C: about 50% of maximal activity, no activity at 60°C Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.143 additional information
-
 poly(ADP-ribose) protects the enzyme from thermal inactivation Bos taurus
3.2.1.143 30
-
 10 min, stable Bos taurus
3.2.1.143 45
-
 10 min, about 10% loss of activity Bos taurus
3.2.1.143 50
-
 10 min, complete inactivation Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.143 90
-
 Poly(ADP-ribose)
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.143 7 7.5
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.143 6 8 about 45% of maximal activity at pH 6.0 and 8.0 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.143 5 9 37°C, 10 min, stable Bos taurus