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Literature summary extracted from

  • Dunn, G.; Manners, D.J.
    The limit dextrinase from ungerminated oats (Avena sativa L.) and ungerminated rice (Oryza sativa L.) (1975), Carbohydr. Res., 39, 283-293.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.142 6,3-alpha-D-glucosylmaltotriose
-
 Oryza sativa
3.2.1.142 6,3-alpha-D-maltosylmaltotetraose
-
 Oryza sativa
3.2.1.142 6,3-alpha-D-maltosylmaltotriose
-
 Oryza sativa
3.2.1.142 6,3-alpha-D-maltotriosylmaltotetraose
-
 Oryza sativa
3.2.1.142 6,3-alpha-D-maltotriosylmaltotriose
-
 Oryza sativa
3.2.1.142 maltose
-
 Oryza sativa
3.2.1.142 oligomaltose
-
 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.142 0.2
-
 6-alpha-maltosylmaltotriose
-
 Avena sativa
3.2.1.142 0.2
-
 6-alpha-maltosylmaltotetraose
-
 Avena sativa
3.2.1.142 0.2
-
 6-alpha-maltotriosylmaltotriose
-
 Avena sativa
3.2.1.142 0.2
-
 6-alpha-maltotriosylmaltotetraose
-
 Avena sativa
3.2.1.142 0.6
-
 6-alpha-maltotriosylmaltotetraose
-
 Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.142 80000
-
 preliminary studies, gel filtration Avena sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.142 polysaccharide + H2O Avena sativa
-
 oligosaccharides
-
 ?
3.2.1.142 polysaccharide + H2O Oryza sativa
-
 oligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.142 Avena sativa
-
 ungerminated oats
-
3.2.1.142 Oryza sativa
-
 ungerminated rice
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.142 acetone precipitation, DEAE-cellulose, Sephadex G-150 Avena sativa
3.2.1.142 acetone precipitation, DEAE-cellulose, Sephadex G-150 Oryza sativa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.142 0.6
-
-
 Oryza sativa
3.2.1.142 0.8
-
-
 Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.142 6-alpha-maltosylmaltotetraose + H2O
-
 Avena sativa ?
-
 ?
3.2.1.142 6-alpha-maltosylmaltotriose + H2O
-
 Avena sativa ?
-
 ?
3.2.1.142 6-alpha-maltotriosylmaltotetraose + H2O
-
 Oryza sativa ?
-
 ?
3.2.1.142 6-alpha-maltotriosylmaltotetraose + H2O
-
 Avena sativa ?
-
 ?
3.2.1.142 polysaccharide + H2O oligosaccharides containing maltosyl or larger side chains are hydrolysed as well as amylopectin and amylopectin beta-limit dextrin, oligo- and poly-saccharides containing single D-glucosyl groups attached by an alpha-(1,6) linkage to the rest of the molecule are not hydrolysed, glycogen is not a substrate Avena sativa oligosaccharides
-
 ?
3.2.1.142 polysaccharide + H2O oligosaccharides containing maltosyl or larger side chains are hydrolysed as well as amylopectin and amylopectin beta-limit dextrin, oligo- and poly-saccharides containing single D-glucosyl groups attached by an alpha-(1,6) linkage to the rest of the molecule are not hydrolysed, glycogen is not a substrate Oryza sativa oligosaccharides
-
 ?
3.2.1.142 polysaccharide + H2O
-
 Avena sativa oligosaccharides
-
 ?
3.2.1.142 polysaccharide + H2O
-
 Oryza sativa oligosaccharides
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.142 5
-
-
 Avena sativa
3.2.1.142 5
-
-
 Oryza sativa