EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.142 | cyclohexaamylose | competitive inhibition | Pisum sativum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.142 | 3.24 | - |
amylopectin beta-limit dextrin | - |
Pisum sativum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.142 | 180000 | - |
gel filtration | Pisum sativum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.142 | polysaccharide + H2O | Pisum sativum | natural substrates appear to be alpha-limit dextrins | oligosaccharides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.142 | Pisum sativum | - |
ungerminated peas | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.142 | acetone precipitation, affinity chromatography | Pisum sativum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.142 | 1.08 | - |
- |
Pisum sativum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.142 | amylopectin beta-limit dextrin + H2O | - |
Pisum sativum | maltotriose + maltose + maltooligosaccharides | - |
? | |
3.2.1.142 | polysaccharide + H2O | oligosaccharides containing maltosyl or larger side chains are hydrolysed as well as amylopectin and amylopectin beta-limit dextrin, oligo- and poly-saccharides containing single D-glucosyl groups attached by an alpha-(1,6) linkage to the rest of the molecule are not hydrolysed, glycogen is not a substrate | Pisum sativum | oligosaccharides | - |
? | |
3.2.1.142 | polysaccharide + H2O | natural substrates appear to be alpha-limit dextrins | Pisum sativum | oligosaccharides | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.142 | 5.8 | 6.2 | with both pullulan and amylopectin beta-limit dextrin as substrates | Pisum sativum |