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Literature summary extracted from

  • Kalinowska, M.; Wojciechowski, Z.A.
    Modulation of activities of steryl glucoside hydrolase and UDPG:sterol glucosyltransferase from Sinapis alba by detergents and lipids (1986), Phytochemistry, 25, 45-49.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.173 Phospholipids not stimulatory Sinapis alba
2.4.1.173 Triton X-100 activity stimulated by 0.1-1% with 6.5fold stimulation at 0.1%, highest activity stimulation with respect to others non-ionic detergents Sinapis alba
2.4.1.173 Tween-20 2fold activity stimulation at 0.1% Sinapis alba
2.4.1.173 Tween-40 70% activity stimulation at 0.05% Sinapis alba
2.4.1.173 Tween-60 35% activity stimulation at 0.1% Sinapis alba
3.2.1.104 cholesteryl acetate 150% stimulation at 0.1 mM Sinapis alba
3.2.1.104 methyl stearate 150% stimulation at 0.1 mM Sinapis alba
3.2.1.104 phytol 200% stimulation at 0.002 mM Sinapis alba
3.2.1.104 Tripalmitoylglycerol 145% stimulation at 0.1 mM Sinapis alba

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.173 sodium deoxycholate strong inhibition at 0.1-1% Sinapis alba
2.4.1.173 sodium taurocholate strong inhibition at 0.1-1% Sinapis alba
3.2.1.104 2-lyso-phosphatidylcholine
-
 Sinapis alba
3.2.1.104 2-lyso-phosphatidylethanolamine
-
 Sinapis alba
3.2.1.104 monogalactosyldiacylglycerol
-
 Sinapis alba
3.2.1.104 phosphatidylcholine
-
 Sinapis alba
3.2.1.104 phosphatidylethanolamine
-
 Sinapis alba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.173 membrane organelles membrane Sinapis alba 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Sinapis alba
-
 white mustard
-
3.2.1.104 Sinapis alba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.173 partial purification by differential centrifugation and precipitation with Me2CO Sinapis alba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 seedling
-
 Sinapis alba
-
3.2.1.104 seedling
-
 Sinapis alba
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.173 -20 C, Tris-HCl 0.1 M, pH 7.2, two weeks Sinapis alba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 UDP-glucose + cholesterol
-
 Sinapis alba UDP + cholesterol 3-beta-D-glucoside
-
 ?
3.2.1.104 cholesteryl beta-D-glucoside + H2O
-
 Sinapis alba D-glucose + cholesterol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.104 SG hydrolase
-
 Sinapis alba

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.173 30
-
 assay at Sinapis alba

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.173 7.2
-
 Tris-HCl 0.1 M Sinapis alba