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Literature summary extracted from

  • Kalinowska, M.; Wojciechowski, Z.A.
    Purification and some properties of steryl beta-D-glucoside hydrolase from Sinapis alba seedlings (1978), Phytochemistry, 17, 1533-1537.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.104 dithiothreitol 45% stimulation at 0.1 mM, hydrolysis of cholesteryl beta-D-glucoside. No effect on hydrolysis of p-nitrophenyl beta-D-glucoside Sinapis alba
3.2.1.104 ethanol 64% stimulation at a concentration of 20%, hydrolysis of cholesteryl beta-D-glucoside. 65% stimulation at a concentration of 20%, hydrolysis of p-nitrophenyl beta-D-glucoside Sinapis alba

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.104 5alpha-cholestanol hydrolysis of cholesteryl glucoside Sinapis alba
3.2.1.104 campesterol hydrolysis of cholesteryl glucoside Sinapis alba
3.2.1.104 cholesterol hydrolysis of cholesteryl glucoside Sinapis alba
3.2.1.104 ethanol 39% inhibition at a concentration of 50%, hydrolysis of p-nitrophenyl beta-D-glucoside Sinapis alba
3.2.1.104 gluconolactone 50% inhibition at 4 mM, hydrolysis of cholesteryl beta-D-glucoside. 50% inhibition at 2 mM, hydrolysis of p-nitrophenyl beta-D-glucoside Sinapis alba
3.2.1.104 sitosterol hydrolysis of cholesteryl glucoside Sinapis alba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.104 cell membrane
-
 Sinapis alba
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.104 65000
-
 gel filtration Sinapis alba

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.104 Sinapis alba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.104 partial Sinapis alba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.104 seedling
-
 Sinapis alba
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.104 0.3621
-
-
 Sinapis alba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.104 cholesteryl beta-D-glucoside + H2O
-
 Sinapis alba D-glucose + cholesterol
-
 ?
3.2.1.104 p-nitrophenyl beta-D-glucoside + H2O
-
 Sinapis alba D-glucose + p-nitrophenol
-
 ?
3.2.1.104 sitosteryl beta-D-glucoside + H2O
-
 Sinapis alba D-glucose + sitosterol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.104 30
-
 hydrolysis of cholesteryl beta-D-glucoside Sinapis alba
3.2.1.104 60
-
 hydrolysis of p-nitrophenyl beta-D-glucoside Sinapis alba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.104 5.2 5.6 hydrolysis of cholesteryl beta-D-glucoside or p-nitrophenyl beta-D-glucoside Sinapis alba