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Literature summary extracted from

  • Kelly, S.J.; Butler, L.G.
    Enzymic hydrolysis of phosphonate esters. Reaction mechanism of intestinal 5'-nucleotide phosphodiesterase (1977), Biochemistry, 16, 1102-1104.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.1 AMP 5'-AMP, competitive Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.1 0.03
-
 NAD+
-
 Bos taurus
3.1.4.1 0.043
-
 p-nitrophenylthymidine 5'-phosphate
-
 Bos taurus
3.1.4.1 0.1
-
 3',5'-cAMP
-
 Bos taurus
3.1.4.1 0.2
-
 ATP
-
 Bos taurus
3.1.4.1 0.85
-
 bis(p-nitrophenyl) phosphate
-
 Bos taurus
3.1.4.1 11
-
 p-Nitrophenyl phenylphosphonate
-
 Bos taurus
3.1.4.1 18
-
 2-naphthyl phenylphosphonate
-
 Bos taurus
3.1.4.1 41
-
 p-nitrophenyl chloromethylphosphonate
-
 Bos taurus
3.1.4.1 66
-
 p-nitrophenyl methylphosphonate
-
 Bos taurus
3.1.4.1 83
-
 2-naphthyl chloromethylphosphonate
-
 Bos taurus
3.1.4.1 120
-
 2-naphthyl methylphosphonate
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Bos taurus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.4.1 [nucleotide]n + H2O = [nucleotide]m + nucleotide mechanism Bos taurus
a
3.1.4.1 [nucleotide]n + H2O = [nucleotide]m + nucleotide ping-pong type mechanism, with participation of a covalent enzyme intermediate Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.1 intestine
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.1 47.7
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 2-naphthyl chloromethylphosphonate + H2O
-
 Bos taurus 2-naphthol + chloromethylphosphonic acid
-
 ?
3.1.4.1 2-naphthyl methylphosphonate + H2O
-
 Bos taurus 2-naphthol + methylphosphonic acid
-
 ?
3.1.4.1 2-naphthyl phenylphosphonate + H2O
-
 Bos taurus 2-naphthol + benzenephosphonic acid
-
 ?
3.1.4.1 ADP + H2O slowly Bos taurus AMP + phosphate
-
 ?
3.1.4.1 ATP + H2O slowly Bos taurus 5'-AMP + diphosphate
-
 ?
3.1.4.1 bis(p-nitrophenyl) phosphate + H2O
-
 Bos taurus ?
-
 ?
3.1.4.1 cAMP + H2O
-
 Bos taurus ?
-
 ?
3.1.4.1 cyclic 3',5'-mononucleotides + H2O 3',5'-cAMP, slowly Bos taurus mononucleoside 5'-phosphate
-
 ?
3.1.4.1 NAD+ + H2O slowly Bos taurus 5'-AMP + NMN
-
 ?
3.1.4.1 p-nitrophenyl chloromethylphosphonate + H2O
-
 Bos taurus p-nitrophenol + chloromethylphosphonic acid
-
 ?
3.1.4.1 p-nitrophenyl methylphosphonate + H2O
-
 Bos taurus p-nitrophenol + methylphosphonic acid
-
 ?
3.1.4.1 p-nitrophenyl phenylphosphonate + H2O
-
 Bos taurus p-nitrophenol + benzenephosphonic acid
-
 ?
3.1.4.1 p-nitrophenylthymidine 5'-phosphate + H2O
-
 Bos taurus 5'-TMP + p-nitrophenol
-
 ?
3.1.4.1 phosphonate ester + H2O
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.1 dimer 2 * ?, identical dimer Bos taurus