BRENDA - Enzyme Database

Enzymic hydrolysis of phosphonate esters. Reaction mechanism of intestinal 5'-nucleotide phosphodiesterase

Kelly, S.J.; Butler, L.G.; Biochemistry 16, 1102-1104 (1977) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.1
AMP
5'-AMP, competitive
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.1
0.03
-
NAD+
-
Bos taurus
3.1.4.1
0.043
-
p-nitrophenylthymidine 5'-phosphate
-
Bos taurus
3.1.4.1
0.1
-
3',5'-cAMP
-
Bos taurus
3.1.4.1
0.2
-
ATP
-
Bos taurus
3.1.4.1
0.85
-
bis(p-nitrophenyl) phosphate
-
Bos taurus
3.1.4.1
11
-
p-Nitrophenyl phenylphosphonate
-
Bos taurus
3.1.4.1
18
-
2-naphthyl phenylphosphonate
-
Bos taurus
3.1.4.1
41
-
p-nitrophenyl chloromethylphosphonate
-
Bos taurus
3.1.4.1
66
-
p-nitrophenyl methylphosphonate
-
Bos taurus
3.1.4.1
83
-
2-naphthyl chloromethylphosphonate
-
Bos taurus
3.1.4.1
120
-
2-naphthyl methylphosphonate
-
Bos taurus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.4.1
Bos taurus
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
3.1.4.1
hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
mechanism
Bos taurus
3.1.4.1
hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
ping-pong type mechanism, with participation of a covalent enzyme intermediate
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.1
intestine
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.1
47.7
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.4.1
2-naphthyl chloromethylphosphonate + H2O
-
208387
Bos taurus
2-naphthol + chloromethylphosphonic acid
-
-
-
?
3.1.4.1
2-naphthyl methylphosphonate + H2O
-
208387
Bos taurus
2-naphthol + methylphosphonic acid
-
-
-
?
3.1.4.1
2-naphthyl phenylphosphonate + H2O
-
208387
Bos taurus
2-naphthol + benzenephosphonic acid
-
-
-
?
3.1.4.1
ADP + H2O
slowly
208387
Bos taurus
AMP + phosphate
-
-
-
?
3.1.4.1
ATP + H2O
slowly
208387
Bos taurus
5'-AMP + diphosphate
-
-
-
?
3.1.4.1
bis(p-nitrophenyl) phosphate + H2O
-
208387
Bos taurus
?
-
-
-
?
3.1.4.1
cAMP + H2O
-
208387
Bos taurus
?
-
-
-
?
3.1.4.1
cyclic 3',5'-mononucleotides + H2O
3',5'-cAMP, slowly
208387
Bos taurus
mononucleoside 5'-phosphate
-
-
-
?
3.1.4.1
NAD+ + H2O
slowly
208387
Bos taurus
5'-AMP + NMN
-
-
-
?
3.1.4.1
p-nitrophenyl chloromethylphosphonate + H2O
-
208387
Bos taurus
p-nitrophenol + chloromethylphosphonic acid
-
-
-
?
3.1.4.1
p-nitrophenyl methylphosphonate + H2O
-
208387
Bos taurus
p-nitrophenol + methylphosphonic acid
-
-
-
?
3.1.4.1
p-nitrophenyl phenylphosphonate + H2O
-
208387
Bos taurus
p-nitrophenol + benzenephosphonic acid
-
-
-
?
3.1.4.1
p-nitrophenylthymidine 5'-phosphate + H2O
-
208387
Bos taurus
5'-TMP + p-nitrophenol
-
-
-
?
3.1.4.1
phosphonate ester + H2O
-
208387
Bos taurus
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.1
dimer
2 * ?, identical dimer
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.1
AMP
5'-AMP, competitive
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.1
0.03
-
NAD+
-
Bos taurus
3.1.4.1
0.043
-
p-nitrophenylthymidine 5'-phosphate
-
Bos taurus
3.1.4.1
0.1
-
3',5'-cAMP
-
Bos taurus
3.1.4.1
0.2
-
ATP
-
Bos taurus
3.1.4.1
0.85
-
bis(p-nitrophenyl) phosphate
-
Bos taurus
3.1.4.1
11
-
p-Nitrophenyl phenylphosphonate
-
Bos taurus
3.1.4.1
18
-
2-naphthyl phenylphosphonate
-
Bos taurus
3.1.4.1
41
-
p-nitrophenyl chloromethylphosphonate
-
Bos taurus
3.1.4.1
66
-
p-nitrophenyl methylphosphonate
-
Bos taurus
3.1.4.1
83
-
2-naphthyl chloromethylphosphonate
-
Bos taurus
3.1.4.1
120
-
2-naphthyl methylphosphonate
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.1
intestine
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.1
47.7
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.4.1
2-naphthyl chloromethylphosphonate + H2O
-
208387
Bos taurus
2-naphthol + chloromethylphosphonic acid
-
-
-
?
3.1.4.1
2-naphthyl methylphosphonate + H2O
-
208387
Bos taurus
2-naphthol + methylphosphonic acid
-
-
-
?
3.1.4.1
2-naphthyl phenylphosphonate + H2O
-
208387
Bos taurus
2-naphthol + benzenephosphonic acid
-
-
-
?
3.1.4.1
ADP + H2O
slowly
208387
Bos taurus
AMP + phosphate
-
-
-
?
3.1.4.1
ATP + H2O
slowly
208387
Bos taurus
5'-AMP + diphosphate
-
-
-
?
3.1.4.1
bis(p-nitrophenyl) phosphate + H2O
-
208387
Bos taurus
?
-
-
-
?
3.1.4.1
cAMP + H2O
-
208387
Bos taurus
?
-
-
-
?
3.1.4.1
cyclic 3',5'-mononucleotides + H2O
3',5'-cAMP, slowly
208387
Bos taurus
mononucleoside 5'-phosphate
-
-
-
?
3.1.4.1
NAD+ + H2O
slowly
208387
Bos taurus
5'-AMP + NMN
-
-
-
?
3.1.4.1
p-nitrophenyl chloromethylphosphonate + H2O
-
208387
Bos taurus
p-nitrophenol + chloromethylphosphonic acid
-
-
-
?
3.1.4.1
p-nitrophenyl methylphosphonate + H2O
-
208387
Bos taurus
p-nitrophenol + methylphosphonic acid
-
-
-
?
3.1.4.1
p-nitrophenyl phenylphosphonate + H2O
-
208387
Bos taurus
p-nitrophenol + benzenephosphonic acid
-
-
-
?
3.1.4.1
p-nitrophenylthymidine 5'-phosphate + H2O
-
208387
Bos taurus
5'-TMP + p-nitrophenol
-
-
-
?
3.1.4.1
phosphonate ester + H2O
-
208387
Bos taurus
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.1
dimer
2 * ?, identical dimer
Bos taurus