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Literature summary extracted from
- Catabolism of Ap3A and Ap4A in human plasma. Purification and characterization of a glycoprotein complex with 5'-nucleotide phosphodiesterase activity (1985), Eur. J. Biochem., 149, 119-127.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.1 | 5'-nucleotides | - |
Homo sapiens |  |
| 3.1.4.1 | ADP | - |
Homo sapiens | |
| 3.1.4.1 | AMP | - |
Homo sapiens | |
| 3.1.4.1 | CMP | - |
Homo sapiens | |
| 3.1.4.1 | CoA | - |
Homo sapiens | |
| 3.1.4.1 | EDTA | completely, inactivation reversed by Ca2+, Co2+ and, at best, with Zn2+ | Homo sapiens | |
| 3.1.4.1 | GTP | - |
Homo sapiens | |
| 3.1.4.1 | Mn2+ | - |
Homo sapiens | |
| 3.1.4.1 | NAD+ | - |
Homo sapiens | |
| 3.1.4.1 | NADH | - |
Homo sapiens | |
| 3.1.4.1 | NADP+ | - |
Homo sapiens | |
| 3.1.4.1 | NADPH | - |
Homo sapiens | |
| 3.1.4.1 | terminal 3'-monophosphoryl group of substrates | - |
Homo sapiens | |
| 3.1.4.1 | UMP | - |
Homo sapiens | |
| 3.6.1.29 | EDTA | - |
Homo sapiens | |
| 3.6.1.29 | Mn2+ | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.1 | 0.0006 | - |
diadenosine 5',5'''-P1,P4-tetraphosphate | Ap4A | Homo sapiens | |
| 3.1.4.1 | 0.001 | - |
diadenosine 5',5'''-P1,P3-triphosphate | Ap3A | Homo sapiens | |
| 3.6.1.29 | 0.0006 | - |
Ap4A | - |
Homo sapiens | |
| 3.6.1.29 | 0.001 | - |
P1-P3-bis(5'-adenosyl) triphosphate | - |
Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.1 | Ca2+ | activation | Homo sapiens | |
| 3.1.4.1 | Co2+ | activation | Homo sapiens | |
| 3.1.4.1 | divalent cations | activation | Homo sapiens | |
| 3.1.4.1 | Mg2+ | activation | Homo sapiens | |
| 3.1.4.1 | Ni2+ | slight activation | Homo sapiens | |
| 3.1.4.1 | Zn2+ | activation | Homo sapiens | |
| 3.1.4.1 | Zn2+ | physiologically bound | Homo sapiens | |
| 3.6.1.29 | Ca2+ | - |
Homo sapiens | |
| 3.6.1.29 | Mg2+ | - |
Homo sapiens | |
| 3.6.1.29 | Zn2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.1 | 16000 | - |
x * 66000 + x * 45000 + x * 16000, enzyme has a complex structure, which is composed of subunits of different size, stoichiometry of the complex is still uncertain, SDS-PAGE | Homo sapiens |
| 3.1.4.1 | 45000 | - |
x * 66000 + x * 45000 + x * 16000, enzyme has a complex structure, which is composed of subunits of different size, stoichiometry of the complex is still uncertain, SDS-PAGE | Homo sapiens |
| 3.1.4.1 | 66000 | - |
x * 66000 + x * 45000 + x * 16000, enzyme has a complex structure, which is composed of subunits of different size, stoichiometry of the complex is still uncertain, SDS-PAGE | Homo sapiens |
| 3.1.4.1 | 230000 | - |
gel filtration | Homo sapiens |
| 3.6.1.29 | 230000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.1 | diadenosine polyphosphate + H2O | Homo sapiens | phosphodiesterase can be considered as a catabolic enzyme | AMP + adenosine polyphosphate-1 | - |
? | |
| 3.1.4.1 | additional information | Homo sapiens | enzyme might function as a modulating factor of platelet aggregation | ? | - |
? | |
| 3.6.1.29 | P1-P3-bis(5'-adenosyl) triphosphate + H2O | Homo sapiens | - |
ADP + AMP | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.1 | Homo sapiens | - |
- |
- |
| 3.6.1.29 | Homo sapiens | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.4.1 | glycoprotein | - |
Homo sapiens |
| 3.6.1.29 | glycoprotein | mannoside | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.1 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.1 | blood plasma | - |
Homo sapiens | - |
| 3.6.1.29 | plasma | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.1 | additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.1 | adenosine 5'-P1-tetraphospho-P4-5'''-adenosine + H2O | - |
Homo sapiens | AMP + ATP | - |
? | |
| 3.1.4.1 | ATP + H2O | - |
Homo sapiens | 5'-AMP + diphosphate | - |
? | |
| 3.1.4.1 | diadenosine 5',5'''-P1,P3-triphosphate + H2O | - |
Homo sapiens | AMP + ADP | - |
? | |
| 3.1.4.1 | diadenosine polyphosphate + H2O | phosphodiesterase can be considered as a catabolic enzyme | Homo sapiens | AMP + adenosine polyphosphate-1 | - |
? | |
| 3.1.4.1 | dinucleotides + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.1.4.1 | additional information | no activity with p-nitrophenylthymidine 3'-phosphate | Homo sapiens | ? | - |
? | |
| 3.1.4.1 | additional information | broad substrate specificity | Homo sapiens | ? | - |
? | |
| 3.1.4.1 | additional information | enzyme might function as a modulating factor of platelet aggregation | Homo sapiens | ? | - |
? | |
| 3.1.4.1 | NAD+ + H2O | - |
Homo sapiens | 5'-AMP + NMN | - |
? | |
| 3.1.4.1 | p-nitrophenylthymidine 5'-phosphate + H2O | - |
Homo sapiens | 5'-TMP + p-nitrophenol | - |
? | |
| 3.1.4.1 | UTP + H2O | - |
Homo sapiens | UMP + diphosphate | - |
? | |
| 3.6.1.29 | Ap4A + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.6.1.29 | ATP + 2 H2O | - |
Homo sapiens | AMP + 2 phosphate | - |
? | |
| 3.6.1.29 | diadenosine 5',5''-P1,P4-tetraphosphate + H2O | - |
Homo sapiens | AMP + ATP | - |
? | |
| 3.6.1.29 | NAD+ + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.6.1.29 | P1-P3-bis(5'-adenosyl) triphosphate + H2O | - |
Homo sapiens | ADP + AMP | - |
? | |
| 3.6.1.29 | thymidine 5'-monophosphate p-nitrophenylester + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.6.1.29 | UTP + 2 H2O | - |
Homo sapiens | UMP + 2 phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.1 | ? | x * 66000 + x * 45000 + x * 16000, enzyme has a complex structure, which is composed of subunits of different size, stoichiometry of the complex is still uncertain, SDS-PAGE | Homo sapiens |
| 3.6.1.29 | polymer | - |
Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.1 | Ap3A (Ap4A) hydrolase | - |
Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.1 | 8 | 9 | - |
Homo sapiens |
| 3.6.1.29 | 8.5 | - |
- |
Homo sapiens |
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