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Literature summary extracted from

  • Uriarte, M.; Stalmans, W.; Hickman, S.; Bollen, M.
    Regulation of purified hepatic PC-1 (phosphodiesterase-I/nucleotide pyrophosphatase) by threonine auto(de)phosphorylation and by binding of acidic fibroblast growth factor (1995), Biochem. J., 306, 271-277.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.1 ADP PC-1 Rattus norvegicus
3.1.4.1 AMP PC-1 Rattus norvegicus
3.1.4.1 ATP PC-1 Rattus norvegicus
3.1.4.1 dithiothreitol PC-1 Rattus norvegicus
3.1.4.1 EDTA PC-1 Rattus norvegicus
3.1.4.1 additional information PC-1, no inhibition by MgCl2 and /or CaCl2 Rattus norvegicus
3.6.1.9 acidic fibroblast growth factor potent inhibition at nearly stoichiometric concentrations, Ki: 1.3 nM Rattus sp.
3.6.1.9 dithiothreitol 50% inhibition at 2.3 mM Rattus sp.
3.6.1.9 EDTA complete inhibition at 1 mM Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.1 cytoplasmic membrane PC-1 Rattus norvegicus
-
-
3.1.4.1 extracellular PC-1, active site of plasma-membrane-associated PC-1 appears to be located extracellularly Rattus norvegicus
-
-
3.6.1.9 membrane
-
 Rattus sp. 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.1 118000
-
 x * 118000 + x * 128000, PC-1, SDS-PAGE Rattus norvegicus
3.1.4.1 128000
-
 x * 118000 + x * 128000, PC-1, SDS-PAGE Rattus norvegicus
3.1.4.1 500000
-
 PC-1, gel filtration Rattus norvegicus
3.6.1.9 118000
-
 2 * 118000 + 2 * 128000, SDS-PAGE Rattus sp.
3.6.1.9 128000
-
 2 * 118000 + 2 * 128000, SDS-PAGE Rattus sp.
3.6.1.9 500000
-
 gel filtration Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.1 additional information Rattus norvegicus PC-1, plasma-membrane-associated enzyme with nucleotide pyrophosphatase/phosphodiesterase I activity is probably involved in the clearance of extracellular nucleotides such as ATP and diadenosine polyphosphates ?
-
 ?
3.6.1.9 ATP + H2O Rattus sp.
-
 AMP + diphosphate little accumulation of ADP ?
3.6.1.9 UDP-glucose + H2O Rattus sp.
-
 UMP + D-glucose-1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Rattus norvegicus
-
 PC-1, nucleotide pyrophosphatase/phosphodiesterase I
-
3.6.1.9 Rattus sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.6.1.9 phosphoprotein autophosphorylation by protein threonin kinase activity with intrinsic dephosphorylase activity Rattus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.1 PC-1, NPP/PDE, nucleotide pyrophosphatase/phosphodiesterase I Rattus norvegicus
3.6.1.9 to homogemeity, chromatography steps, trifunctional enzyme, displaying phosphodiesterase-1/diphosphatase- and protein threonin kinase activity, that also phosphorylates histone IIa Rattus sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.4.1 hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides PC-1, enzymic activities and regulation of PC-1 Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.1 liver PC-1, nucleotide pyrophosphatase/phosphodiesterase I Rattus norvegicus
-
3.6.1.9 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.1 411
-
 PC-1, phosphodiesterase I activity, substrate: p-nitrophenylthymidine 5'-phosphate Rattus norvegicus
3.6.1.9 411
-
 phosphodiesterase activity Rattus sp.

Storage Stability

EC Number Storage Stability Organism
3.1.4.1 PC-1, -20øC, 150 mM NaCl Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 ATP + H2O PC-1 Rattus norvegicus 5'-AMP + diphosphate PC-1 ?
3.1.4.1 additional information PC-1, plasma-membrane-associated enzyme with nucleotide pyrophosphatase/phosphodiesterase I activity is probably involved in the clearance of extracellular nucleotides such as ATP and diadenosine polyphosphates Rattus norvegicus ?
-
 ?
3.1.4.1 p-nitrophenylthymidine 5'-phosphate + H2O PC-1 Rattus norvegicus 5'-TMP + p-nitrophenol
-
 ?
3.1.4.1 UDP-glucose + H2O PC-1 Rattus norvegicus glucose-1-phosphate + 5'-UMP PC-1 ?
3.6.1.9 ATP + H2O
-
 Rattus sp. AMP + diphosphate little accumulation of ADP ?
3.6.1.9 thymidine 5'-monophosphate p-nitrophenyl ester + H2O significant phosphodiesterase activity Rattus sp. thymidine 5'-monophosphate + p-nitrophenol
-
 ?
3.6.1.9 UDP-glucose + H2O
-
 Rattus sp. UMP + D-glucose-1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.1 tetramer x * 118000 + x * 128000, PC-1, SDS-PAGE Rattus norvegicus
3.6.1.9 tetramer 2 * 118000 + 2 * 128000, SDS-PAGE Rattus sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.4.1 PC-1 PC-1, plasma-cell differentiation antigen-1, a trifunctional enzyme, displaying a phosphodiesterase I /nucleotide pyrophosphatase activity, a protein threonine kinase activity, and a nucleotide-stimulated protein threonine phosphatase activity Rattus norvegicus
3.6.1.9 nucleotide pyrophosphatase/phosphodiesterase-I
-
 Rattus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.9 0.0000013
-
 acidic fibroblast growth factor potent inhibition at nearly stoichiometric concentrations Rattus sp.
3.6.1.9 2.3
-
 dithiothreitol
-
 Rattus sp.