EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.1 | ADP | PC-1 | Rattus norvegicus | |
3.1.4.1 | AMP | PC-1 | Rattus norvegicus | |
3.1.4.1 | ATP | PC-1 | Rattus norvegicus | |
3.1.4.1 | dithiothreitol | PC-1 | Rattus norvegicus | |
3.1.4.1 | EDTA | PC-1 | Rattus norvegicus | |
3.1.4.1 | additional information | PC-1, no inhibition by MgCl2 and /or CaCl2 | Rattus norvegicus | |
3.6.1.9 | acidic fibroblast growth factor | potent inhibition at nearly stoichiometric concentrations, Ki: 1.3 nM | Rattus sp. | |
3.6.1.9 | dithiothreitol | 50% inhibition at 2.3 mM | Rattus sp. | |
3.6.1.9 | EDTA | complete inhibition at 1 mM | Rattus sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.4.1 | cytoplasmic membrane | PC-1 | Rattus norvegicus | - |
- |
3.1.4.1 | extracellular | PC-1, active site of plasma-membrane-associated PC-1 appears to be located extracellularly | Rattus norvegicus | - |
- |
3.6.1.9 | membrane | - |
Rattus sp. | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.4.1 | 118000 | - |
x * 118000 + x * 128000, PC-1, SDS-PAGE | Rattus norvegicus |
3.1.4.1 | 128000 | - |
x * 118000 + x * 128000, PC-1, SDS-PAGE | Rattus norvegicus |
3.1.4.1 | 500000 | - |
PC-1, gel filtration | Rattus norvegicus |
3.6.1.9 | 118000 | - |
2 * 118000 + 2 * 128000, SDS-PAGE | Rattus sp. |
3.6.1.9 | 128000 | - |
2 * 118000 + 2 * 128000, SDS-PAGE | Rattus sp. |
3.6.1.9 | 500000 | - |
gel filtration | Rattus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.1 | additional information | Rattus norvegicus | PC-1, plasma-membrane-associated enzyme with nucleotide pyrophosphatase/phosphodiesterase I activity is probably involved in the clearance of extracellular nucleotides such as ATP and diadenosine polyphosphates | ? | - |
? | |
3.6.1.9 | ATP + H2O | Rattus sp. | - |
AMP + diphosphate | little accumulation of ADP | ? | |
3.6.1.9 | UDP-glucose + H2O | Rattus sp. | - |
UMP + D-glucose-1-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.1 | Rattus norvegicus | - |
PC-1, nucleotide pyrophosphatase/phosphodiesterase I | - |
3.6.1.9 | Rattus sp. | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.6.1.9 | phosphoprotein | autophosphorylation by protein threonin kinase activity with intrinsic dephosphorylase activity | Rattus sp. |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.1 | PC-1, NPP/PDE, nucleotide pyrophosphatase/phosphodiesterase I | Rattus norvegicus |
3.6.1.9 | to homogemeity, chromatography steps, trifunctional enzyme, displaying phosphodiesterase-1/diphosphatase- and protein threonin kinase activity, that also phosphorylates histone IIa | Rattus sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.4.1 | [nucleotide]n + H2O = [nucleotide]m + nucleotide | PC-1, enzymic activities and regulation of PC-1 | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.4.1 | liver | PC-1, nucleotide pyrophosphatase/phosphodiesterase I | Rattus norvegicus | - |
3.6.1.9 | liver | - |
Rattus sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.4.1 | 411 | - |
PC-1, phosphodiesterase I activity, substrate: p-nitrophenylthymidine 5'-phosphate | Rattus norvegicus |
3.6.1.9 | 411 | - |
phosphodiesterase activity | Rattus sp. |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.4.1 | PC-1, -20øC, 150 mM NaCl | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.1 | ATP + H2O | PC-1 | Rattus norvegicus | 5'-AMP + diphosphate | PC-1 | ? | |
3.1.4.1 | additional information | PC-1, plasma-membrane-associated enzyme with nucleotide pyrophosphatase/phosphodiesterase I activity is probably involved in the clearance of extracellular nucleotides such as ATP and diadenosine polyphosphates | Rattus norvegicus | ? | - |
? | |
3.1.4.1 | p-nitrophenylthymidine 5'-phosphate + H2O | PC-1 | Rattus norvegicus | 5'-TMP + p-nitrophenol | - |
? | |
3.1.4.1 | UDP-glucose + H2O | PC-1 | Rattus norvegicus | glucose-1-phosphate + 5'-UMP | PC-1 | ? | |
3.6.1.9 | ATP + H2O | - |
Rattus sp. | AMP + diphosphate | little accumulation of ADP | ? | |
3.6.1.9 | thymidine 5'-monophosphate p-nitrophenyl ester + H2O | significant phosphodiesterase activity | Rattus sp. | thymidine 5'-monophosphate + p-nitrophenol | - |
? | |
3.6.1.9 | UDP-glucose + H2O | - |
Rattus sp. | UMP + D-glucose-1-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.1 | tetramer | x * 118000 + x * 128000, PC-1, SDS-PAGE | Rattus norvegicus |
3.6.1.9 | tetramer | 2 * 118000 + 2 * 128000, SDS-PAGE | Rattus sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.1 | PC-1 | PC-1, plasma-cell differentiation antigen-1, a trifunctional enzyme, displaying a phosphodiesterase I /nucleotide pyrophosphatase activity, a protein threonine kinase activity, and a nucleotide-stimulated protein threonine phosphatase activity | Rattus norvegicus |
3.6.1.9 | nucleotide pyrophosphatase/phosphodiesterase-I | - |
Rattus sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.9 | 0.0000013 | - |
acidic fibroblast growth factor | potent inhibition at nearly stoichiometric concentrations | Rattus sp. | |
3.6.1.9 | 2.3 | - |
dithiothreitol | - |
Rattus sp. |