Data extracted from this reference:
Activating Compound
3.1.4.1
nicotinamide
patients with neurofibromatosis, induction of alkaline phosphodiesterase I
Homo sapiens
General Stability
3.1.4.1
enzyme extracted with Triton-X-100 is unstable
Homo sapiens
3.1.4.1
glycerol, 15%, stabilizes
Homo sapiens
3.1.4.1
Zn2+ stabilizes
Homo sapiens
Inhibitors
3.1.4.1
dithiothreitol
Homo sapiens
3.1.4.1
EDTA
Homo sapiens
3.1.4.1
GTP
competitive
Homo sapiens
Localization
3.1.4.1
cytoplasmic membrane
Homo sapiens
Metals/Ions
3.1.4.1
Ca2+
slight activation
Homo sapiens
3.1.4.1
Mg2+
slight activation
Homo sapiens
3.1.4.1
Zn2+
required for activity
Homo sapiens
Molecular Weight [Da]
3.1.4.1
125000
x * 125000, SDS-PAGE
Homo sapiens
3.1.4.1
430000
gel filtration
Homo sapiens
Organism
3.1.4.1
Homo sapiens
patients with neurofibromatosis: over-expression of alkaline phosphodiesterase I
Purification (Commentary)
3.1.4.1
partial, patients with neurofibromatosis
Homo sapiens
Source Tissue
3.1.4.1
fibroblast
tumor-derived and normal fibroblasts, patients with neurofibromatosis
Homo sapiens
Specific Activity [micromol/min/mg]
Substrates and Products (Substrate)
3.1.4.1
DNA + H2O
enzyme hydolyzes single-stranded DNA more preferentially than double-stranded DNA, the enzyme also hydrolyzes nicked superhelical covalently closed circular phiX174RFI DNA to yield first open circular DNA and then linear DNA
208373
Homo sapiens
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
?
3.1.4.1
GTP + H2O
208373
Homo sapiens
?
?
3.1.4.1
p-nitrophenylthymidine 5'-phosphate + H2O
208373
Homo sapiens
5'-TMP + p-nitrophenol
?
Subunits
3.1.4.1
?
x * 125000, SDS-PAGE
Homo sapiens
Synonyms
3.1.4.1
alkaline phosphodiesterase
alkaline phosphodiesterase I
Homo sapiens
Temperature Stability [°C]
3.1.4.1
additional information
enzyme very heat labile without Zn2+
Homo sapiens
3.1.4.1
60
enzyme preincubated with Zn2+, 50% of activity remained at, in the absence of Zn2+, no activity remained at
Homo sapiens
Activating Compound (protein specific)
3.1.4.1
nicotinamide
patients with neurofibromatosis, induction of alkaline phosphodiesterase I
Homo sapiens
General Stability (protein specific)
3.1.4.1
enzyme extracted with Triton-X-100 is unstable
Homo sapiens
3.1.4.1
glycerol, 15%, stabilizes
Homo sapiens
3.1.4.1
Zn2+ stabilizes
Homo sapiens
Inhibitors (protein specific)
3.1.4.1
dithiothreitol
Homo sapiens
3.1.4.1
EDTA
Homo sapiens
3.1.4.1
GTP
competitive
Homo sapiens
Localization (protein specific)
3.1.4.1
cytoplasmic membrane
Homo sapiens
Metals/Ions (protein specific)
3.1.4.1
Ca2+
slight activation
Homo sapiens
3.1.4.1
Mg2+
slight activation
Homo sapiens
3.1.4.1
Zn2+
required for activity
Homo sapiens
Molecular Weight [Da] (protein specific)
3.1.4.1
125000
x * 125000, SDS-PAGE
Homo sapiens
3.1.4.1
430000
gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
3.1.4.1
partial, patients with neurofibromatosis
Homo sapiens
Source Tissue (protein specific)
3.1.4.1
fibroblast
tumor-derived and normal fibroblasts, patients with neurofibromatosis
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
Substrates and Products (Substrate) (protein specific)
3.1.4.1
DNA + H2O
enzyme hydolyzes single-stranded DNA more preferentially than double-stranded DNA, the enzyme also hydrolyzes nicked superhelical covalently closed circular phiX174RFI DNA to yield first open circular DNA and then linear DNA
208373
Homo sapiens
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
?
3.1.4.1
GTP + H2O
208373
Homo sapiens
?
?
3.1.4.1
p-nitrophenylthymidine 5'-phosphate + H2O
208373
Homo sapiens
5'-TMP + p-nitrophenol
?
Subunits (protein specific)
3.1.4.1
?
x * 125000, SDS-PAGE
Homo sapiens
Temperature Stability [°C] (protein specific)
3.1.4.1
additional information
enzyme very heat labile without Zn2+
Homo sapiens
3.1.4.1
60
enzyme preincubated with Zn2+, 50% of activity remained at, in the absence of Zn2+, no activity remained at
Homo sapiens