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Literature summary extracted from

  • Maruyama, E.; Takashima, S.
    Characterization of over-expressed alkaline phosphodiesterase I in tumor-derived fibroblasts from patients with neurofibromatosis (1993), Cell Biochem. Funct., 11, 271-277.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.1 nicotinamide patients with neurofibromatosis, induction of alkaline phosphodiesterase I Homo sapiens

General Stability

EC Number General Stability Organism
3.1.4.1 enzyme extracted with Triton-X-100 is unstable Homo sapiens
3.1.4.1 glycerol, 15%, stabilizes Homo sapiens
3.1.4.1 Zn2+ stabilizes Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.1 dithiothreitol
-
 Homo sapiens
3.1.4.1 EDTA
-
 Homo sapiens
3.1.4.1 GTP competitive Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.1 cytoplasmic membrane
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.1 Ca2+ slight activation Homo sapiens
3.1.4.1 Mg2+ slight activation Homo sapiens
3.1.4.1 Zn2+ required for activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.1 125000
-
 x * 125000, SDS-PAGE Homo sapiens
3.1.4.1 430000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Homo sapiens
-
 patients with neurofibromatosis: over-expression of alkaline phosphodiesterase I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.1 partial, patients with neurofibromatosis Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.1 fibroblast tumor-derived and normal fibroblasts, patients with neurofibromatosis Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.1 1.1
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 DNA + H2O enzyme hydolyzes single-stranded DNA more preferentially than double-stranded DNA, the enzyme also hydrolyzes nicked superhelical covalently closed circular phiX174RFI DNA to yield first open circular DNA and then linear DNA Homo sapiens DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
 ?
3.1.4.1 GTP + H2O
-
 Homo sapiens ?
-
 ?
3.1.4.1 p-nitrophenylthymidine 5'-phosphate + H2O
-
 Homo sapiens 5'-TMP + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.1 ? x * 125000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.4.1 alkaline phosphodiesterase alkaline phosphodiesterase I Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.1 additional information
-
 enzyme very heat labile without Zn2+ Homo sapiens
3.1.4.1 60
-
 enzyme preincubated with Zn2+, 50% of activity remained at, in the absence of Zn2+, no activity remained at Homo sapiens