BRENDA - Enzyme Database show

Characterization of over-expressed alkaline phosphodiesterase I in tumor-derived fibroblasts from patients with neurofibromatosis

Maruyama, E.; Takashima, S.; Cell Biochem. Funct. 11, 271-277 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.1
nicotinamide
patients with neurofibromatosis, induction of alkaline phosphodiesterase I
Homo sapiens
General Stability
EC Number
General Stability
Organism
3.1.4.1
enzyme extracted with Triton-X-100 is unstable
Homo sapiens
3.1.4.1
glycerol, 15%, stabilizes
Homo sapiens
3.1.4.1
Zn2+ stabilizes
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.1
dithiothreitol
-
Homo sapiens
3.1.4.1
EDTA
-
Homo sapiens
3.1.4.1
GTP
competitive
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.1
cytoplasmic membrane
-
Homo sapiens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.1
Ca2+
slight activation
Homo sapiens
3.1.4.1
Mg2+
slight activation
Homo sapiens
3.1.4.1
Zn2+
required for activity
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.1
125000
-
x * 125000, SDS-PAGE
Homo sapiens
3.1.4.1
430000
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.1
Homo sapiens
-
patients with neurofibromatosis: over-expression of alkaline phosphodiesterase I
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.1
partial, patients with neurofibromatosis
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.1
fibroblast
tumor-derived and normal fibroblasts, patients with neurofibromatosis
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.1
1.1
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.1
DNA + H2O
enzyme hydolyzes single-stranded DNA more preferentially than double-stranded DNA, the enzyme also hydrolyzes nicked superhelical covalently closed circular phiX174RFI DNA to yield first open circular DNA and then linear DNA
208373
Homo sapiens
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
-
-
?
3.1.4.1
GTP + H2O
-
208373
Homo sapiens
?
-
-
-
?
3.1.4.1
p-nitrophenylthymidine 5'-phosphate + H2O
-
208373
Homo sapiens
5'-TMP + p-nitrophenol
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.1
?
x * 125000, SDS-PAGE
Homo sapiens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.1
additional information
-
enzyme very heat labile without Zn2+
Homo sapiens
3.1.4.1
60
-
enzyme preincubated with Zn2+, 50% of activity remained at, in the absence of Zn2+, no activity remained at
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.1
nicotinamide
patients with neurofibromatosis, induction of alkaline phosphodiesterase I
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.1
enzyme extracted with Triton-X-100 is unstable
Homo sapiens
3.1.4.1
glycerol, 15%, stabilizes
Homo sapiens
3.1.4.1
Zn2+ stabilizes
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.1
dithiothreitol
-
Homo sapiens
3.1.4.1
EDTA
-
Homo sapiens
3.1.4.1
GTP
competitive
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.1
cytoplasmic membrane
-
Homo sapiens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.1
Ca2+
slight activation
Homo sapiens
3.1.4.1
Mg2+
slight activation
Homo sapiens
3.1.4.1
Zn2+
required for activity
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.1
125000
-
x * 125000, SDS-PAGE
Homo sapiens
3.1.4.1
430000
-
gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.1
partial, patients with neurofibromatosis
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.1
fibroblast
tumor-derived and normal fibroblasts, patients with neurofibromatosis
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.1
1.1
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.1
DNA + H2O
enzyme hydolyzes single-stranded DNA more preferentially than double-stranded DNA, the enzyme also hydrolyzes nicked superhelical covalently closed circular phiX174RFI DNA to yield first open circular DNA and then linear DNA
208373
Homo sapiens
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
-
-
?
3.1.4.1
GTP + H2O
-
208373
Homo sapiens
?
-
-
-
?
3.1.4.1
p-nitrophenylthymidine 5'-phosphate + H2O
-
208373
Homo sapiens
5'-TMP + p-nitrophenol
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.1
?
x * 125000, SDS-PAGE
Homo sapiens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.1
additional information
-
enzyme very heat labile without Zn2+
Homo sapiens
3.1.4.1
60
-
enzyme preincubated with Zn2+, 50% of activity remained at, in the absence of Zn2+, no activity remained at
Homo sapiens