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Literature summary extracted from

  • Wang, A.Y.; Juang, R.H.; Chang, C.T.; Sung, H.Y.
    Purification and characterization of 5'-phosphodiesterase from barley rootlets (1993), Biochem. Mol. Biol. Int., 29, 1095-1102.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.1 2-mercaptoethanol
-
 Hordeum vulgare
3.1.4.1 5'-deoxyribonucleotides end-product inhibition Hordeum vulgare
3.1.4.1 5'-nucleotides end-product inhibition Hordeum vulgare
3.1.4.1 5'-ribonucleotides end-product inhibition Hordeum vulgare
3.1.4.1 Cu2+
-
 Hordeum vulgare
3.1.4.1 CuSO4
-
 Hordeum vulgare
3.1.4.1 EDTA
-
 Hordeum vulgare
3.1.4.1 glutathione
-
 Hordeum vulgare
3.1.4.1 KF slight inhibition Hordeum vulgare
3.1.4.1 L-cysteine
-
 Hordeum vulgare
3.1.4.1 Reducing agents
-
 Hordeum vulgare
3.1.4.1 Zn2+
-
 Hordeum vulgare
3.1.4.1 ZnSO4 slight inhibition Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.1 0.28
-
 p-nitrophenylthymidine 5'-phosphate
-
 Hordeum vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.1 (NH4)2SO4 slight activation Hordeum vulgare
3.1.4.1 Ca2+ slight activation Hordeum vulgare
3.1.4.1 divalent cations required for activity Hordeum vulgare
3.1.4.1 K+ slight activation Hordeum vulgare
3.1.4.1 KCl slight activation Hordeum vulgare
3.1.4.1 Mg2+ activation Hordeum vulgare
3.1.4.1 MgCl2 activation Hordeum vulgare
3.1.4.1 Na+ slight activation Hordeum vulgare
3.1.4.1 NaCl slight activation Hordeum vulgare
3.1.4.1 NH4Cl slight activation Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.1 52000
-
 52000 + 34000 + 16000, PDE might be a dimer with two 52000 subunits, or a heterologous trimer composed of 52000, 34000 and 16000 subunits, in the former case, one of the subunits is nicked and produced the 34000 + 16000 subunits, SDS-PAGE Hordeum vulgare
3.1.4.1 100000
-
 gel filtration and PAGE Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.1 additional information Hordeum vulgare the physiological function of PDE in plant cell is presumably the degradation of nucleic acids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Hordeum vulgare
-
 barley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.1
-
 Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.1 root rootlet Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.1 51.8
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 DNA + H2O denatured DNA. Native DNA is resistant Hordeum vulgare DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
 ?
3.1.4.1 additional information the physiological function of PDE in plant cell is presumably the degradation of nucleic acids Hordeum vulgare ?
-
 ?
3.1.4.1 oligonucleotides + H2O specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner Hordeum vulgare oligonucleotides(n-1) + 5'-mononucleotides
-
 ?
3.1.4.1 p-nitrophenylthymidine 5'-phosphate + H2O
-
 Hordeum vulgare 5'-TMP + p-nitrophenol
-
 ?
3.1.4.1 polynucleotides + H2O specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner Hordeum vulgare polynucleotides(n-1) + 5'-mononucleotides
-
 ?
3.1.4.1 RNA + H2O
-
 Hordeum vulgare RNA(n-1) + nucleoside 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.1 dimer 52000 + 34000 + 16000, PDE might be a dimer with two 52000 subunits, or a heterologous trimer composed of 52000, 34000 and 16000 subunits, in the former case, one of the subunits is nicked and produced the 34000 + 16000 subunits, SDS-PAGE Hordeum vulgare
3.1.4.1 trimer 52000 + 34000 + 16000, PDE might be a dimer with two 52000 subunits, or a heterologous trimer composed of 52000, 34000 and 16000 subunits, in the former case, one of the subunits is nicked and produced the 34000 + 16000 subunits, SDS-PAGE Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
3.1.4.1 5'-PDE
-
 Hordeum vulgare
3.1.4.1 PDE I
-
 Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.1 9.5
-
-
 Hordeum vulgare