BRENDA - Enzyme Database

The synthesis of 4-methylumbelliferyl phenylphosphonate and its use in an improved method for the zymogram analysis of phosphodiesterase I

Hawley, D.M.; Crisp, M.; Hodes, M.E.; Anal. Biochem. 129, 522-528 (1983) View publication on PubMed

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.1
0.2
-
p-nitrophenylthymidine 5'-phosphate
-
Bos taurus
3.1.4.1
0.22
-
4-methylumbelliferyl thymidine 5'-phosphate
-
Bos taurus
3.1.4.1
2.7
-
bis(p-nitrophenyl) phosphate
-
Bos taurus
3.1.4.1
8.2
-
4-methylumbelliferyl phenylphosphonate
-
Bos taurus
3.1.4.1
34.6
-
p-Nitrophenyl phenylphosphonate
-
Bos taurus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.4.1
Bos taurus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.1
intestine
intestinal mucosa
Bos taurus
-
3.1.4.1
mucosa
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.4.1
2-naphthyl phenylphosphonate + H2O
-
208369
Bos taurus
2-naphthol + benzenephosphonic acid
-
-
-
?
3.1.4.1
4-methylumbelliferyl phenylphosphonate + H2O
-
208369
Bos taurus
4-methylumbelliferone + phenylphosphonic acid
-
-
-
?
3.1.4.1
4-methylumbelliferyl thymidine 5'-phosphate + H2O
-
208369
Bos taurus
4-methylumbelliferone + 5'-TMP
-
-
-
?
3.1.4.1
bis(p-nitrophenyl) phosphate + H2O
-
208369
Bos taurus
?
-
-
-
?
3.1.4.1
p-nitrophenyl phenylphosphonate + H2O
-
208369
Bos taurus
p-nitrophenol + benzenephosphonic acid
-
-
-
?
3.1.4.1
p-nitrophenylthymidine 5'-phosphate + H2O
-
208369
Bos taurus
5'-TMP + p-nitrophenol
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.1.4.1
PDE I
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.1
0.2
-
p-nitrophenylthymidine 5'-phosphate
-
Bos taurus
3.1.4.1
0.22
-
4-methylumbelliferyl thymidine 5'-phosphate
-
Bos taurus
3.1.4.1
2.7
-
bis(p-nitrophenyl) phosphate
-
Bos taurus
3.1.4.1
8.2
-
4-methylumbelliferyl phenylphosphonate
-
Bos taurus
3.1.4.1
34.6
-
p-Nitrophenyl phenylphosphonate
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.1
intestine
intestinal mucosa
Bos taurus
-
3.1.4.1
mucosa
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.4.1
2-naphthyl phenylphosphonate + H2O
-
208369
Bos taurus
2-naphthol + benzenephosphonic acid
-
-
-
?
3.1.4.1
4-methylumbelliferyl phenylphosphonate + H2O
-
208369
Bos taurus
4-methylumbelliferone + phenylphosphonic acid
-
-
-
?
3.1.4.1
4-methylumbelliferyl thymidine 5'-phosphate + H2O
-
208369
Bos taurus
4-methylumbelliferone + 5'-TMP
-
-
-
?
3.1.4.1
bis(p-nitrophenyl) phosphate + H2O
-
208369
Bos taurus
?
-
-
-
?
3.1.4.1
p-nitrophenyl phenylphosphonate + H2O
-
208369
Bos taurus
p-nitrophenol + benzenephosphonic acid
-
-
-
?
3.1.4.1
p-nitrophenylthymidine 5'-phosphate + H2O
-
208369
Bos taurus
5'-TMP + p-nitrophenol
-
-
-
?