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Literature summary extracted from

  • Razzell, W.E.
    Partial purification and properties of phosphodiesterase I from hog kidney (1961), J. Biol. Chem., 236, 3031-3036.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.1 5'-deoxyribonucleotides end-product inhibition Sus scrofa
3.1.4.1 5'-nucleotides end-product inhibition Sus scrofa
3.1.4.1 ADP competitive Sus scrofa
3.1.4.1 AMP product inhibition Sus scrofa
3.1.4.1 ATP competitive Sus scrofa
3.1.4.1 Cu2+ purified enzyme Sus scrofa
3.1.4.1 EDTA
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.1 0.046
-
 p-nitrophenylthymidine 5'-phosphate pH 9.1 Sus scrofa
3.1.4.1 0.17
-
 p-nitrophenylthymidine 5'-phosphate pH 8.0 Sus scrofa
3.1.4.1 0.26
-
 3'-O-acetyl-p-nitrophenylthymidine 5'-phosphate pH 8.0 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.1 lysosome
-
 Sus scrofa 5764
-
3.1.4.1 microsome
-
 Sus scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.1 Mg2+ slight activation Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.1 partial, kidney Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.1 kidney
-
 Sus scrofa
-
3.1.4.1 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.1 15.5 29
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 3'-O-acetyl-5'-O-[hydroxy(4-nitrophenoxy)phosphoryl]thymidine + H2O
-
 Sus scrofa p-nitrophenol + 3'-O-acetylthymidine 5'-phosphate
-
 ?
3.1.4.1 5'-p-nitrophenyluridine 5'-phosphate + H2O 70% as fast as 5'-p-nitrophenylthymidine 5'-phosphate Sus scrofa 5'-UMP + p-nitrophenol
-
 ?
3.1.4.1 ADP + H2O slowly Sus scrofa AMP + phosphate
-
 ?
3.1.4.1 ATP + H2O slowly Sus scrofa 5'-AMP + diphosphate
-
 ?
3.1.4.1 cyclic 3',5'-mononucleotides + H2O slowly Sus scrofa mononucleoside 5'-phosphate
-
 ?
3.1.4.1 additional information no activity with dinucleotides bearing 3'-phosphate Sus scrofa ?
-
 ?
3.1.4.1 additional information no activity with p-nitrophenylthymidine 3'-phosphate Sus scrofa ?
-
 ?
3.1.4.1 oligonucleotides + H2O specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner Sus scrofa oligonucleotides(n-1) + 5'-mononucleotides
-
 ?
3.1.4.1 p-nitrophenylthymidine 5'-phosphate + H2O
-
 Sus scrofa 5'-TMP + p-nitrophenol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.1 55 65
-
 Sus scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.4.1 35 70
-
 Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.1 66
-
 stable below Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.1 9 9.1
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.1 7.5 10.5
-
 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.1 5
-
 inactivation below Sus scrofa
3.1.4.1 6 10 stable between Sus scrofa