Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lerch, B.; Wolf, G.
    Isolation of phosphodiesterase from sugar beet leaves (1972), Biochim. Biophys. Acta, 258, 206-218.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.1 2-mercaptoethanol
-
 Beta vulgaris
3.1.4.1 5'-deoxyribonucleotides end-product inhibition Beta vulgaris
3.1.4.1 5'-nucleotides end-product inhibition Beta vulgaris
3.1.4.1 5'-ribonucleotides end-product inhibition Beta vulgaris
3.1.4.1 AMP product inhibition Beta vulgaris
3.1.4.1 ascorbic acid slight inhibition Beta vulgaris
3.1.4.1 EDTA
-
 Beta vulgaris
3.1.4.1 glutathione
-
 Beta vulgaris
3.1.4.1 Hg2+
-
 Beta vulgaris
3.1.4.1 L-cysteine
-
 Beta vulgaris
3.1.4.1 Reducing agents
-
 Beta vulgaris
3.1.4.1 terminal 3'-monophosphoryl group of substrates
-
 Beta vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.1 0.19
-
 4-nitrophenyl uridine 5'-phosphate
-
 Beta vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.1 Ca2+ slight activation Beta vulgaris
3.1.4.1 Co2+ activation Beta vulgaris
3.1.4.1 Cu2+ activation Beta vulgaris
3.1.4.1 divalent cations activation Beta vulgaris
3.1.4.1 Mg2+ slight activation Beta vulgaris
3.1.4.1 Mn2+ activation Beta vulgaris
3.1.4.1 Zn2+ activation Beta vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.1 110000
-
 gel filtration Beta vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Beta vulgaris
-
 var. saccharifera, sugar beet
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.1
-
 Beta vulgaris

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.4.1 [nucleotide]n + H2O = [nucleotide]m + nucleotide enzyme is an exonuclease which needs a free end of an oligonucleotide chain for activity Beta vulgaris
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.1 leaf
-
 Beta vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.1 125.2
-
-
 Beta vulgaris

Storage Stability

EC Number Storage Stability Organism
3.1.4.1 frozen, several months without loss of activity Beta vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 4-nitrophenyl uridine 5'-phosphate + H2O
-
 Beta vulgaris 4-nitrophenol + uridine 5'-phosphate
-
 ?
3.1.4.1 5'-p-nitrophenyl deoxythymidine 5'-phosphate + H2O
-
 Beta vulgaris 5'-dTMP + p-nitrophenol
-
 ?
3.1.4.1 5'-p-nitrophenylnucleoside 5'-monophosphate + H2O
-
 Beta vulgaris nucleoside 5'-monophosphate + p-nitrophenol
-
 ?
3.1.4.1 ADP + H2O
-
 Beta vulgaris AMP + phosphate
-
 ?
3.1.4.1 ATP + H2O
-
 Beta vulgaris 5'-AMP + diphosphate
-
 ?
3.1.4.1 DNA + H2O denatured DNA. Native DNA is resistant Beta vulgaris DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
 ?
3.1.4.1 additional information no activity with the cyclic trimer of deoxythymidine 5'-phosphate and adenosine 3',5'-monophosphate Beta vulgaris ?
-
 ?
3.1.4.1 NAD+ + H2O
-
 Beta vulgaris 5'-AMP + NMN
-
 ?
3.1.4.1 oligonucleotides + H2O
-
 Beta vulgaris oligonucleotides(n-1) + 5'-mononucleotides
-
 ?
3.1.4.1 p-nitrophenylthymidine 5'-phosphate + H2O
-
 Beta vulgaris 5'-TMP + p-nitrophenol
-
 ?
3.1.4.1 poly A + H2O
-
 Beta vulgaris 5'-AMP
-
 ?
3.1.4.1 poly C + H2O
-
 Beta vulgaris 5'-CMP
-
 ?
3.1.4.1 poly U + H2O
-
 Beta vulgaris 5'-UMP
-
 ?
3.1.4.1 poly(I) + H2O
-
 Beta vulgaris 5'-IMP
-
 ?
3.1.4.1 polynucleotides + H2O
-
 Beta vulgaris polynucleotides(n-1) + 5'-mononucleotides
-
 ?
3.1.4.1 RNA + H2O
-
 Beta vulgaris RNA(n-1) + nucleoside 5'-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.1 80
-
 up to Beta vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.1 9.2
-
-
 Beta vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.1 5
-
 above, loss in activity below pH 5 Beta vulgaris
3.1.4.1 7.9 10.5
-
 Beta vulgaris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.1 5
-
 inactivation below, unstable near isoelectric point of 3.85 Beta vulgaris
3.1.4.1 9
-
 stable at Beta vulgaris