BRENDA - Enzyme Database

Three diadenosine 5',5'''-P1,P4-tetraphosphate hydrolytic enzymes from Physarum polycephalum with differential effects by calcium: a specific dinucleoside polyphosphate pyrophosphohydrolase, a nucleotide pyrophosphatase, and a phosphodiesterase

Robinson, A.K.; Barnes, L.D.; Arch. Biochem. Biophys. 248, 502-515 (1986)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.1
EGTA
-
Physarum polycephalum
3.1.4.1
Zn2+
-
Physarum polycephalum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.1
Ca2+
activation, 50-60 times more stimulatory than Mg2+ or Mn2+
Physarum polycephalum
3.1.4.1
Co2+
no significant effect
Physarum polycephalum
3.1.4.1
divalent cations
activation
Physarum polycephalum
3.1.4.1
Mg2+
slight activation
Physarum polycephalum
3.1.4.1
Mn2+
slight activation
Physarum polycephalum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.1
45300
-
gel filtration
Physarum polycephalum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.1
Physarum polycephalum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.1
partial
Physarum polycephalum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.1
plasmodium
microplasmodium
Physarum polycephalum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.1
0.169
-
-
Physarum polycephalum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.1
adenosine 5'-P1-tetraphospho-P4-5'''-adenosine + H2O
-
208360
Physarum polycephalum
AMP + ATP
-
-
-
?
3.1.4.1
adenosine 5'-P1-tetraphospho-P4-5'''-guanosine + H2O
-
208360
Physarum polycephalum
?
-
-
-
?
3.1.4.1
ADP + H2O
-
208360
Physarum polycephalum
AMP + phosphate
-
-
-
?
3.1.4.1
ATP + H2O
-
208360
Physarum polycephalum
5'-AMP + diphosphate
-
-
-
?
3.1.4.1
bis(p-nitrophenyl) phosphate + H2O
-
208360
Physarum polycephalum
?
-
-
-
?
3.1.4.1
dinucleoside polyphosphate + H2O
-
208360
Physarum polycephalum
?
-
-
-
?
3.1.4.1
additional information
broad substrate specificity
208360
Physarum polycephalum
?
-
-
-
-
3.1.4.1
NADP+ + H2O
-
208360
Physarum polycephalum
NAD+ + phosphate
-
208360
Physarum polycephalum
?
3.1.4.1
p-nitrophenyl phenylphosphonate + H2O
-
208360
Physarum polycephalum
p-nitrophenol + benzenephosphonic acid
-
-
-
?
3.1.4.1
p-nitrophenylthymidine 5'-phosphate + H2O
-
208360
Physarum polycephalum
5'-TMP + p-nitrophenol
-
-
-
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.1
EGTA
-
Physarum polycephalum
3.1.4.1
Zn2+
-
Physarum polycephalum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.1
Ca2+
activation, 50-60 times more stimulatory than Mg2+ or Mn2+
Physarum polycephalum
3.1.4.1
Co2+
no significant effect
Physarum polycephalum
3.1.4.1
divalent cations
activation
Physarum polycephalum
3.1.4.1
Mg2+
slight activation
Physarum polycephalum
3.1.4.1
Mn2+
slight activation
Physarum polycephalum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.1
45300
-
gel filtration
Physarum polycephalum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.1
partial
Physarum polycephalum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.1
plasmodium
microplasmodium
Physarum polycephalum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.1
0.169
-
-
Physarum polycephalum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.1
adenosine 5'-P1-tetraphospho-P4-5'''-adenosine + H2O
-
208360
Physarum polycephalum
AMP + ATP
-
-
-
?
3.1.4.1
adenosine 5'-P1-tetraphospho-P4-5'''-guanosine + H2O
-
208360
Physarum polycephalum
?
-
-
-
?
3.1.4.1
ADP + H2O
-
208360
Physarum polycephalum
AMP + phosphate
-
-
-
?
3.1.4.1
ATP + H2O
-
208360
Physarum polycephalum
5'-AMP + diphosphate
-
-
-
?
3.1.4.1
bis(p-nitrophenyl) phosphate + H2O
-
208360
Physarum polycephalum
?
-
-
-
?
3.1.4.1
dinucleoside polyphosphate + H2O
-
208360
Physarum polycephalum
?
-
-
-
?
3.1.4.1
additional information
broad substrate specificity
208360
Physarum polycephalum
?
-
-
-
-
3.1.4.1
NADP+ + H2O
-
208360
Physarum polycephalum
NAD+ + phosphate
-
208360
Physarum polycephalum
?
3.1.4.1
p-nitrophenyl phenylphosphonate + H2O
-
208360
Physarum polycephalum
p-nitrophenol + benzenephosphonic acid
-
-
-
?
3.1.4.1
p-nitrophenylthymidine 5'-phosphate + H2O
-
208360
Physarum polycephalum
5'-TMP + p-nitrophenol
-
-
-
?