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Literature summary extracted from

  • Takeda, M.; Koizumi, J.I.; Yabe, K.; Adachi, K.
    Thermostable poly(3-hydroxybutyrate) depolymerase of a thermophilic strain of Leptothrix sp. isolated from a hot spring (1998), J. Ferment. Bioeng., 85, 375-380.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.75 dithiothreitol
-
 Leptothrix sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.75 additional information
-
 poly(3-hydroxybutanoate)n 0.018 mg/ml Leptothrix sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Leptothrix sp. (in: Bacteria)
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.75 104000
-
 gel filtration Leptothrix sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Leptothrix sp. (in: Bacteria)
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Leptothrix sp. (in: Bacteria) HS
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Leptothrix sp. (in: Bacteria)
-
-
-
3.1.1.75 Leptothrix sp. (in: Bacteria) HS
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 to homogeneity, single step chromatography Leptothrix sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.75 cell culture
-
 Leptothrix sp. (in: Bacteria)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 poly(3-hydroxybutyric acid) + H2O
-
 Leptothrix sp. (in: Bacteria) ?
-
 ?
3.1.1.75 poly(3-hydroxybutyric acid) + H2O
-
 Leptothrix sp. (in: Bacteria) HS ?
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O
-
 Leptothrix sp. (in: Bacteria) poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O
-
 Leptothrix sp. (in: Bacteria) HS poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.75 monomer 1 * 104000, SDS-PAGE Leptothrix sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.75 70
-
-
 Leptothrix sp. (in: Bacteria)