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Literature summary extracted from

  • Purdy, R.E.; Kolattukudy, P.E.
    Hydrolysis of plant cuticle by plant pathogens. Properties of cutinase I, cutinase II, and a nonspecific esterase isolated from Fusarium solani pisi (1975), Biochemistry, 14, 2832-2840.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.74 crystallization of native enzyme, 27 mutant enzymes and 4 covalently inhibited complexes, crystallizes in 8 different crystal forms Fusarium solani

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.74 diisopropyl fluorophosphate
-
 Fusarium solani
3.1.1.74 paraoxon
-
 Fusarium solani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.74 0.35
-
 p-nitrophenylbutanoate cutinase I Fusarium solani
3.1.1.74 0.36
-
 p-nitrophenyldecanoate cutinase II Fusarium solani
3.1.1.74 0.45
-
 p-nitrophenyldodecanoate cutinase II Fusarium solani
3.1.1.74 0.48
-
 p-nitrophenyldecanoate cutinase I Fusarium solani
3.1.1.74 0.56
-
 p-nitrophenyldodecanoate cutinase I Fusarium solani
3.1.1.74 0.59
-
 4-nitrophenyl octanoate cutinase II Fusarium solani
3.1.1.74 0.75
-
 p-nitrophenylbutanoate cutinase II Fusarium solani
3.1.1.74 0.86
-
 4-nitrophenyl hexanoate cutinase II Fusarium solani
3.1.1.74 0.88
-
 4-nitrophenyl octanoate cutinase I Fusarium solani
3.1.1.74 0.89
-
 4-nitrophenyl hexanoate cutinase I Fusarium solani
3.1.1.74 6.8
-
 4-nitrophenyl acetate cutinase I Fusarium solani
3.1.1.74 9.7
-
 4-nitrophenyl acetate cutinase II Fusarium solani

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.74 extracellular
-
 Fusarium solani
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.74 20400
-
 cutinase II, equilibrium sedimentation Fusarium solani
3.1.1.74 21400
-
 1 * 21400, cutinase I and II, SDS-PAGE Fusarium solani
3.1.1.74 21800
-
 cutinase I and II, gel filtration Fusarium solani
3.1.1.74 23400
-
 cutinase I, equilibrium sedimentation Fusarium solani

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.74 Fusarium solani
-
 cutinase I and cutinase II
-
3.1.1.74 Fusarium solani
-
 pisi
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.74 culture medium
-
 Fusarium solani
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.74 4-nitrophenyl acetate + H2O
-
 Fusarium solani 4-nitrophenol + acetate
-
 ?
3.1.1.74 4-nitrophenyl dodecanoate + H2O
-
 Fusarium solani 4-nitrophenol + dodecanoate
-
 ?
3.1.1.74 4-nitrophenyl octanoate + H2O
-
 Fusarium solani 4-nitrophenol + octanoate
-
 ?
3.1.1.74 cutin + H2O
-
 Fusarium solani additional information dihydroxyhexadecanoic acid, cutin monomer + cutin oligomers ?
3.1.1.74 cyclohexyl hexadecanoate + H2O
-
 Fusarium solani hexadecanoic acid + cyclohexanol
-
 ?
3.1.1.74 hexadecyl hexadecanoate + H2O weak activity Fusarium solani hexadecanoic acid + hexadecanol
-
 ?
3.1.1.74 methyl hexadecanoate + H2O
-
 Fusarium solani hexadecanoic acid + methanol
-
 ?
3.1.1.74 p-nitrophenyl acetate + H2O
-
 Fusarium solani p-nitrophenol + acetate
-
 ?
3.1.1.74 p-nitrophenyl hexanoate + H2O
-
 Fusarium solani p-nitrophenol + hexanoate
-
 ?
3.1.1.74 p-nitrophenylbutanoate + H2O
-
 Fusarium solani p-nitrophenol + butanoate
-
 ?
3.1.1.74 p-nitrophenyldecanoate + H2O
-
 Fusarium solani p-nitrophenol + decanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.74 ? x * 21800, cutinase I, SDS-PAGE Fusarium solani
3.1.1.74 ? x * 21800, and a smaller band of 10600 Da, cutinase II, SDS-PAGE Fusarium solani
3.1.1.74 monomer 1 * 21400, cutinase I and II, SDS-PAGE Fusarium solani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.74 10
-
-
 Fusarium solani