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Literature summary extracted from

  • Dzedzyulya, E.I.; Becker, E.G.; Okunev, O.N.; Sinitsyn, A.P.
    Feruloyl esterase from Aspergillus sp.: Purification, properties, and action on natural substrates (1999), Biochemistry (Moscow), 64, 405-410.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Aspergillus sp.
-
-
3.1.1.73 extracellular
-
 Aspergillus awamori
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 42000
-
-
 Aspergillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus niger
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus sp.
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus awamori
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 sugar beet pulp + H2O Aspergillus niger
-
 ferulic acid + ?
-
 ?
3.1.1.73 sugar beet pulp + H2O Aspergillus sp.
-
 ferulic acid + ?
-
 ?
3.1.1.73 wheat bran + H2O Aspergillus niger
-
 ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 wheat bran + H2O Aspergillus sp.
-
 ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 wheat bran + H2O Aspergillus awamori
-
 ferulic acid + p-coumaric acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus awamori
-
-
-
3.1.1.73 Aspergillus niger
-
-
-
3.1.1.73 Aspergillus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
 Aspergillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 1.23
-
 activity toward ferulic acid methyl ester Aspergillus sp.
3.1.1.73 26
-
 activity toward feruloyl arabinoside Aspergillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 1-naphthyl acetate + H2O
-
 Aspergillus sp. acetic acid + 1-naphthol
-
 ?
3.1.1.73 1-naphthyl acetate + H2O
-
 Aspergillus awamori acetic acid + 1-naphthol
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Aspergillus niger ferulic acid + methanol
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Aspergillus sp. ferulic acid + methanol
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Aspergillus awamori ferulic acid + methanol
-
 ?
3.1.1.73 feruloyl arabinoside + H2O structural analog of natural substrates of feruloyl esterases Aspergillus sp. ferulic acid + coumaric acid + cinnamic acid + arabinose
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus niger ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus sp. ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus awamori ferulate + polysaccharide
-
 ?
3.1.1.73 additional information
-
 Aspergillus niger ?
-
 ?
3.1.1.73 additional information
-
 Aspergillus awamori ?
-
 ?
3.1.1.73 additional information does not hydrolyze wheat straw, purified enzyme is inactive towards the p-coumaric methyl ester Aspergillus sp. ?
-
 ?
3.1.1.73 p-coumaric acid methyl ester + H2O
-
 Aspergillus niger p-coumaric acid + methanol
-
 ?
3.1.1.73 p-coumaric acid methyl ester + H2O
-
 Aspergillus sp. p-coumaric acid + methanol
-
 ?
3.1.1.73 p-coumaric acid methyl ester + H2O
-
 Aspergillus awamori p-coumaric acid + methanol
-
 ?
3.1.1.73 p-nitrophenyl acetate + H2O
-
 Aspergillus niger p-nitrophenol + acetate
-
 ?
3.1.1.73 p-nitrophenyl acetate + H2O
-
 Aspergillus sp. p-nitrophenol + acetate
-
 ?
3.1.1.73 p-nitrophenyl acetate + H2O
-
 Aspergillus awamori p-nitrophenol + acetate
-
 ?
3.1.1.73 sugar beet pulp + H2O
-
 Aspergillus niger ferulic acid + ?
-
 ?
3.1.1.73 sugar beet pulp + H2O
-
 Aspergillus sp. ferulic acid + ?
-
 ?
3.1.1.73 wheat bran + H2O
-
 Aspergillus niger ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 wheat bran + H2O
-
 Aspergillus sp. ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 wheat bran + H2O
-
 Aspergillus awamori ferulic acid + p-coumaric acid + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 40
-
 maximal activity toward ferulic acid methyl ester Aspergillus sp.
3.1.1.73 50 60 maximal activity toward feruloyl arabinoside Aspergillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 50
-
-
 Aspergillus awamori
3.1.1.73 50
-
 completely inactivated within 30 min at 70°C Aspergillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 4 6
-
 Aspergillus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 5
-
-
 Aspergillus sp.