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Literature summary extracted from

  • McCrae, S.I.; Leith, K.M.; Gordon, A.H.; Wood, T.M.
    Xylan-degrading enzyme system produced by the fungus Aspergillus awamori: isolation and characterization of a feruloyl esterase and a p-coumaroyl esterase (1994), Enzyme Microb. Technol., 16, 826-834.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.73 Ag+
-
 Aspergillus awamori
3.1.1.73 Ba2+ inhibits feruloyl esterase, no effect on p-coumaroyl esterase Aspergillus awamori
3.1.1.73 Fe2+
-
 Aspergillus awamori
3.1.1.73 Fe2+
-
 Talaromyces pinophilus
3.1.1.73 Hg2+
-
 Aspergillus awamori
3.1.1.73 Zn2+ inhibits feruloyl esterase, no effect on p-coumaroyl esterase Aspergillus awamori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.031
-
 methyl p-coumarate
-
 Aspergillus awamori
3.1.1.73 0.14
-
 ferulic acid methyl ester
-
 Talaromyces pinophilus
3.1.1.73 0.93
-
 methyl ferulate
-
 Aspergillus awamori
3.1.1.73 1.86
-
 methyl ferulate
-
 Streptomyces olivochromogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Streptomyces olivochromogenes
-
-
3.1.1.73 extracellular
-
 Aspergillus awamori
-
-
3.1.1.73 extracellular
-
 Talaromyces pinophilus
-
-
3.1.1.73 extracellular
-
 Neocallimastix sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 11000
-
 p-coumaroyl esterase Neocallimastix sp.
3.1.1.73 24000
-
-
 Neocallimastix sp.
3.1.1.73 29000
-
-
 Streptomyces olivochromogenes
3.1.1.73 30000
-
-
 Aspergillus oryzae
3.1.1.73 57000
-
-
 Talaromyces pinophilus
3.1.1.73 69000
-
-
 Neocallimastix sp.
3.1.1.73 75000
-
 p-coumaroyl esterase, SDS-PAGE Aspergillus awamori
3.1.1.73 112000
-
 ferruloyl esterase, SDS-PAGE Aspergillus awamori
3.1.1.73 132000
-
-
 Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 feruloyl xylooligosaccharide + H2O Streptomyces olivochromogenes
-
 ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O Aspergillus oryzae
-
 ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O Aspergillus phoenicis
-
 ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O Fibrobacter succinogenes
-
 ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O Aspergillus awamori
-
 ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O Talaromyces pinophilus
-
 ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O Neocallimastix sp.
-
 ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus niger
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Streptomyces olivochromogenes
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus oryzae
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus phoenicis
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Fibrobacter succinogenes
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Schizophyllum commune
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Aspergillus awamori
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Streptomyces viridosporus
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Talaromyces pinophilus
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Neocallimastix sp.
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 italian rye gras cell walls + H2O Aspergillus awamori
-
 ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 italian rye gras cell walls + H2O Talaromyces pinophilus
-
 ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 maize internodes + H2O Aspergillus awamori
-
 ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 oat straw + H2O Aspergillus awamori
-
 ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 wheat bran + H2O Aspergillus niger
-
 ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 wheat bran + H2O Aspergillus awamori
-
 ferulic acid + p-coumaric acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus awamori
-
-
-
3.1.1.73 Aspergillus awamori IMI 142717
-
-
-
3.1.1.73 Aspergillus niger
-
-
-
3.1.1.73 Aspergillus oryzae
-
-
-
3.1.1.73 Aspergillus phoenicis
-
-
-
3.1.1.73 Fibrobacter succinogenes
-
-
-
3.1.1.73 Neocallimastix sp.
-
-
-
3.1.1.73 Schizophyllum commune
-
-
-
3.1.1.73 Streptomyces olivochromogenes
-
-
-
3.1.1.73 Streptomyces viridosporus
-
-
-
3.1.1.73 Talaromyces pinophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
 Aspergillus awamori

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 0.39
-
 p-coumaroyl Aspergillus awamori
3.1.1.73 2.5
-
 feruoyl esterase Aspergillus awamori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus awamori 4-nitrophenol + D-glucopyranose
-
 ?
3.1.1.73 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus awamori IMI 142717 4-nitrophenol + D-glucopyranose
-
 ?
3.1.1.73 acetylxylan + H2O
-
 Aspergillus awamori acetic acid + ?
-
 ?
3.1.1.73 acetylxylan + H2O
-
 Talaromyces pinophilus acetic acid + ?
-
 ?
3.1.1.73 acetylxylan + H2O
-
 Aspergillus awamori IMI 142717 acetic acid + ?
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Aspergillus niger ferulic acid + methanol
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Streptomyces olivochromogenes ferulic acid + methanol
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Aspergillus awamori ferulic acid + methanol
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Talaromyces pinophilus ferulic acid + methanol
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O
-
 Neocallimastix sp. ferulic acid + methanol
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O
-
 Streptomyces olivochromogenes ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O
-
 Aspergillus oryzae ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O
-
 Aspergillus phoenicis ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O
-
 Fibrobacter succinogenes ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O
-
 Aspergillus awamori ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O
-
 Talaromyces pinophilus ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O
-
 Neocallimastix sp. ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus niger ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Streptomyces olivochromogenes ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus oryzae ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus phoenicis ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Fibrobacter succinogenes ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Schizophyllum commune ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Aspergillus awamori ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Streptomyces viridosporus ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Talaromyces pinophilus ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Neocallimastix sp. ferulate + polysaccharide
-
 ?
3.1.1.73 italian rye gras cell walls + H2O
-
 Aspergillus awamori ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 italian rye gras cell walls + H2O
-
 Talaromyces pinophilus ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 maize internodes + H2O
-
 Aspergillus awamori ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 additional information
-
 Aspergillus niger ?
-
 ?
3.1.1.73 additional information
-
 Aspergillus awamori ?
-
 ?
3.1.1.73 additional information
-
 Talaromyces pinophilus ?
-
 ?
3.1.1.73 additional information
-
 Neocallimastix sp. ?
-
 ?
3.1.1.73 additional information unable to attack feruloylated xylan in bran Streptomyces olivochromogenes ?
-
 ?
3.1.1.73 additional information
-
 Aspergillus awamori IMI 142717 ?
-
 ?
3.1.1.73 O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAXX Aspergillus awamori ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
 ?
3.1.1.73 O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAXX Neocallimastix sp. ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
 ?
3.1.1.73 oat straw + H2O
-
 Aspergillus awamori ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 p-coumaric acid methyl ester + H2O
-
 Aspergillus niger p-coumaric acid + methanol
-
 ?
3.1.1.73 p-coumaric acid methyl ester + H2O
-
 Streptomyces olivochromogenes p-coumaric acid + methanol
-
 ?
3.1.1.73 p-coumaric acid methyl ester + H2O
-
 Aspergillus awamori p-coumaric acid + methanol
-
 ?
3.1.1.73 p-coumaric acid methyl ester + H2O
-
 Talaromyces pinophilus p-coumaric acid + methanol
-
 ?
3.1.1.73 p-nitrophenyl acetate + H2O
-
 Aspergillus niger p-nitrophenol + acetate
-
 ?
3.1.1.73 p-nitrophenyl acetate + H2O
-
 Aspergillus awamori p-nitrophenol + acetate
-
 ?
3.1.1.73 p-nitrophenyl acetate + H2O
-
 Talaromyces pinophilus p-nitrophenol + acetate
-
 ?
3.1.1.73 p-nitrophenyl acetate + H2O
-
 Aspergillus awamori IMI 142717 p-nitrophenol + acetate
-
 ?
3.1.1.73 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus awamori p-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.1.1.73 wheat bran + H2O
-
 Aspergillus niger ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 wheat bran + H2O
-
 Aspergillus awamori ferulic acid + p-coumaric acid + ?
-
 ?
3.1.1.73 xylan polysaccharide + H2O
-
 Aspergillus niger ferulic acid + p-coumaric acid
-
 ?
3.1.1.73 xylan polysaccharide + H2O
-
 Aspergillus oryzae ferulic acid + p-coumaric acid
-
 ?
3.1.1.73 xylan polysaccharide + H2O
-
 Aspergillus awamori ferulic acid + p-coumaric acid
-
 ?
3.1.1.73 xylan polysaccharide + H2O
-
 Talaromyces pinophilus ferulic acid + p-coumaric acid
-
 ?
3.1.1.73 xylan polysaccharide + H2O
-
 Aspergillus awamori IMI 142717 ferulic acid + p-coumaric acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 dimer
-
 Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 xylan-degrading enzyme system
-
 Aspergillus awamori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 50
-
-
 Aspergillus awamori
3.1.1.73 50
-
-
 Neocallimastix sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 30 60
-
 Aspergillus awamori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 50 80 loses most of its activity when heated for 10 min at 70°C Aspergillus awamori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5
-
-
 Aspergillus niger
3.1.1.73 5
-
 both feruloyl esterase and p-coumaroyl esterase Aspergillus awamori
3.1.1.73 5.5
-
-
 Streptomyces olivochromogenes
3.1.1.73 5.5
-
 p-coumaroyl esterase Streptomyces viridosporus
3.1.1.73 6
-
-
 Aspergillus niger
3.1.1.73 6
-
-
 Talaromyces pinophilus
3.1.1.73 6.2 7 feruloyl esterase Neocallimastix sp.
3.1.1.73 7.5
-
 p-coumaroyl esterase Neocallimastix sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 3 8.1
-
 Aspergillus awamori