EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.73 | EDTA | 1 mM markedly increases activity, 5 mM slightly inhibitory | Talaromyces pinophilus |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.73 | degradation | selective modification of xylans, degradation may be commercially important | Talaromyces pinophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.73 | Cu2+ | - |
Talaromyces pinophilus | |
3.1.1.73 | ethanol | slightly inhibitory at a concentration of 10% | Talaromyces pinophilus | |
3.1.1.73 | Fe2+ | - |
Aspergillus awamori | |
3.1.1.73 | Fe2+ | - |
Talaromyces pinophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 0.08 | - |
p-coumaric acid methyl ester | - |
Talaromyces pinophilus | |
3.1.1.73 | 0.14 | - |
ferulic acid methyl ester | - |
Talaromyces pinophilus | |
3.1.1.73 | 1.86 | - |
methyl ferulate | - |
Streptomyces olivochromogenes |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.73 | extracellular | - |
Streptomyces olivochromogenes | - |
- |
3.1.1.73 | extracellular | - |
Aspergillus awamori | - |
- |
3.1.1.73 | extracellular | - |
Talaromyces pinophilus | - |
- |
3.1.1.73 | extracellular | - |
Neocallimastix sp. | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.73 | Ag2+ | stimulates activity | Talaromyces pinophilus | |
3.1.1.73 | Ca2+ | stimulates activity | Talaromyces pinophilus | |
3.1.1.73 | Mg2+ | stimulates activity | Talaromyces pinophilus | |
3.1.1.73 | Zn2+ | stimulates activity | Talaromyces pinophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 11000 | - |
- |
Neocallimastix sp. |
3.1.1.73 | 29000 | - |
- |
Streptomyces olivochromogenes |
3.1.1.73 | 57000 | - |
SDS-PAGE | Talaromyces pinophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | feruloyl xylooligosaccharide + H2O | Aspergillus awamori | - |
ferulic acid + xylooligosaccharide | - |
? | |
3.1.1.73 | feruloyl xylooligosaccharide + H2O | Talaromyces pinophilus | only feruloylated xylooligomers with specific structures are substrates | ferulic acid + xylooligosaccharide | - |
? | |
3.1.1.73 | feruloyl xylooligosaccharide + H2O | Streptomyces olivochromogenes | esterified xylooligomers generated by xylanase action | ferulic acid + xylooligosaccharide | - |
? | |
3.1.1.73 | feruloyl xylooligosaccharide + H2O | Aspergillus oryzae | esterified xylooligomers generated by xylanase action | ferulic acid + xylooligosaccharide | - |
? | |
3.1.1.73 | feruloyl xylooligosaccharide + H2O | Neocallimastix sp. | esterified xylooligomers generated by xylanase action | ferulic acid + xylooligosaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | Streptomyces olivochromogenes | - |
ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | Aspergillus oryzae | - |
ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | Schizophyllum commune | - |
ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | Aspergillus awamori | - |
ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | Talaromyces pinophilus | - |
ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | Neocallimastix sp. | - |
ferulate + polysaccharide | - |
? | |
3.1.1.73 | italian rye gras cell walls + H2O | Aspergillus awamori | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
3.1.1.73 | italian rye gras cell walls + H2O | Talaromyces pinophilus | - |
ferulic acid + p-coumaric acid + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.73 | Aspergillus awamori | - |
- |
- |
3.1.1.73 | Aspergillus oryzae | - |
- |
- |
3.1.1.73 | Neocallimastix sp. | - |
rumen fungus, MC-2 | - |
3.1.1.73 | Schizophyllum commune | - |
- |
- |
3.1.1.73 | Streptomyces olivochromogenes | - |
- |
- |
3.1.1.73 | Talaromyces pinophilus | - |
CMI 87160 ii | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.73 | - |
Talaromyces pinophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 28.4 | - |
ferulic acid methyl ester as substrate | Talaromyces pinophilus |
3.1.1.73 | 33.7 | - |
p-coumaric acid methyl ester | Talaromyces pinophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | 1-naphthyl acetate + H2O | - |
Aspergillus awamori | acetic acid + 1-naphthol | - |
? | |
3.1.1.73 | 1-naphthyl acetate + H2O | - |
Talaromyces pinophilus | acetic acid + 1-naphthol | - |
? | |
3.1.1.73 | acetylxylan + H2O | - |
Aspergillus awamori | acetic acid + ? | - |
? | |
3.1.1.73 | acetylxylan + H2O | - |
Talaromyces pinophilus | acetic acid + ? | - |
? | |
3.1.1.73 | de-starched wheat bran + H2O | - |
Streptomyces olivochromogenes | ferulic acid + ? | - |
? | |
3.1.1.73 | de-starched wheat bran + H2O | - |
Schizophyllum commune | ferulic acid + ? | - |
? | |
3.1.1.73 | de-starched wheat bran + H2O | - |
Talaromyces pinophilus | ferulic acid + ? | - |
? | |
3.1.1.73 | ferulic acid methyl ester + H2O | - |
Streptomyces olivochromogenes | ferulic acid + methanol | - |
? | |
3.1.1.73 | ferulic acid methyl ester + H2O | - |
Aspergillus awamori | ferulic acid + methanol | - |
? | |
3.1.1.73 | ferulic acid methyl ester + H2O | - |
Talaromyces pinophilus | ferulic acid + methanol | - |
? | |
3.1.1.73 | ferulic acid methyl ester + H2O | - |
Neocallimastix sp. | ferulic acid + methanol | - |
? | |
3.1.1.73 | feruloyl xylooligosaccharide + H2O | - |
Aspergillus awamori | ferulic acid + xylooligosaccharide | - |
? | |
3.1.1.73 | feruloyl xylooligosaccharide + H2O | only feruloylated xylooligomers with specific structures are substrates | Talaromyces pinophilus | ferulic acid + xylooligosaccharide | - |
? | |
3.1.1.73 | feruloyl xylooligosaccharide + H2O | esterified xylooligomers generated by xylanase action | Streptomyces olivochromogenes | ferulic acid + xylooligosaccharide | - |
? | |
3.1.1.73 | feruloyl xylooligosaccharide + H2O | esterified xylooligomers generated by xylanase action | Aspergillus oryzae | ferulic acid + xylooligosaccharide | - |
? | |
3.1.1.73 | feruloyl xylooligosaccharide + H2O | esterified xylooligomers generated by xylanase action | Neocallimastix sp. | ferulic acid + xylooligosaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Streptomyces olivochromogenes | ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Aspergillus oryzae | ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Schizophyllum commune | ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Aspergillus awamori | ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Talaromyces pinophilus | ferulate + polysaccharide | - |
? | |
3.1.1.73 | feruloyl-polysaccharide + H2O | - |
Neocallimastix sp. | ferulate + polysaccharide | - |
? | |
3.1.1.73 | italian rye gras cell walls + H2O | - |
Aspergillus awamori | ferulic acid + p-coumaric acid + ? | - |
? | |
3.1.1.73 | italian rye gras cell walls + H2O | - |
Talaromyces pinophilus | ferulic acid + p-coumaric acid + ? | - |
? | |
3.1.1.73 | additional information | - |
Streptomyces olivochromogenes | ? | - |
? | |
3.1.1.73 | additional information | - |
Aspergillus awamori | ? | - |
? | |
3.1.1.73 | additional information | - |
Neocallimastix sp. | ? | - |
? | |
3.1.1.73 | additional information | no capacity to release ferulic and p-coumaric acid from grass cell wall preparations in absence of other xylanolytic enzymes, methyl umbelliferyltrimethyl ammonium cinnamate is no substrate | Talaromyces pinophilus | ? | - |
? | |
3.1.1.73 | O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAXX | Aspergillus awamori | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
3.1.1.73 | O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAXX | Neocallimastix sp. | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
3.1.1.73 | O-5-O-[(E)-p-coumaroyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | PAXX | Neocallimastix sp. | p-coumaric acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
3.1.1.73 | p-coumaric acid methyl ester + H2O | - |
Streptomyces olivochromogenes | p-coumaric acid + methanol | - |
? | |
3.1.1.73 | p-coumaric acid methyl ester + H2O | - |
Aspergillus awamori | p-coumaric acid + methanol | - |
? | |
3.1.1.73 | p-coumaric acid methyl ester + H2O | - |
Talaromyces pinophilus | p-coumaric acid + methanol | - |
? | |
3.1.1.73 | p-nitrophenyl acetate + H2O | - |
Aspergillus awamori | p-nitrophenol + acetate | - |
? | |
3.1.1.73 | p-nitrophenyl acetate + H2O | - |
Talaromyces pinophilus | p-nitrophenol + acetate | - |
? | |
3.1.1.73 | xylan polysaccharide + H2O | - |
Aspergillus oryzae | ferulic acid + p-coumaric acid | - |
? | |
3.1.1.73 | xylan polysaccharide + H2O | - |
Aspergillus awamori | ferulic acid + p-coumaric acid | - |
? | |
3.1.1.73 | xylan polysaccharide + H2O | water-soluble wheat straw xylan, enzyme is extremely active on esterified xylan polysaccharide | Talaromyces pinophilus | ferulic acid + p-coumaric acid | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.73 | monomer | - |
Aspergillus awamori |
3.1.1.73 | monomer | - |
Neocallimastix sp. |
3.1.1.73 | monomer | 1 * 29000, SDS-PAGE | Streptomyces olivochromogenes |
3.1.1.73 | monomer | 1 * 57000, SDS-PAGE | Talaromyces pinophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 55 | - |
methyl ester of ferulic acid or methyl ester of p-coumaric acid as substrate | Talaromyces pinophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 37 | 70 | - |
Talaromyces pinophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 37 | 55 | very stable in this range for up to 8h, at 55°C for 24 h and at 60°C for 2 h still retains 20% original activity, inactive at 60°C for 4 h | Talaromyces pinophilus |
3.1.1.73 | 37 | 60 | - |
Talaromyces pinophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 6 | - |
- |
Talaromyces pinophilus |