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Literature summary extracted from

  • Niimura, T.; Tokiedo, T.; Yamaha, T.
    Partial purification and some properties of cyclamate sulfamatase (1974), J. Biochem., 75, 407-417.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.10.1.2 gelatin is effective stabilizer Pseudomonas sp.
3.10.1.2 in Tris buffer minor loss of activity than in phosphate buffer Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.10.1.2 (NH4)2SO4
-
 Pseudomonas sp.
3.10.1.2 BaCl2
-
 Pseudomonas sp.
3.10.1.2 Cd2+
-
 Pseudomonas sp.
3.10.1.2 CoSO4
-
 Pseudomonas sp.
3.10.1.2 CuSO4
-
 Pseudomonas sp.
3.10.1.2 Cyclohexylamine
-
 Pseudomonas sp.
3.10.1.2 EDTA metal ions reverse inhibition Pseudomonas sp.
3.10.1.2 H3BO3
-
 Pseudomonas sp.
3.10.1.2 Hg2+
-
 Pseudomonas sp.
3.10.1.2 iodoacetic acid
-
 Pseudomonas sp.
3.10.1.2 MgSO4
-
 Pseudomonas sp.
3.10.1.2 MnCl2
-
 Pseudomonas sp.
3.10.1.2 Na2B4O7
-
 Pseudomonas sp.
3.10.1.2 Na2CO3
-
 Pseudomonas sp.
3.10.1.2 Na2SO3
-
 Pseudomonas sp.
3.10.1.2 NaN3
-
 Pseudomonas sp.
3.10.1.2 NH2OH
-
 Pseudomonas sp.
3.10.1.2 NiSO4
-
 Pseudomonas sp.
3.10.1.2 p-chloromercuribenzoate
-
 Pseudomonas sp.
3.10.1.2 phosphate
-
 Pseudomonas sp.
3.10.1.2 potassium citrate
-
 Pseudomonas sp.
3.10.1.2 ZnSO4
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.10.1.2 5
-
 cyclamate
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.10.1.2 Ca2+ stimulation Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.10.1.2 Pseudomonas sp.
-
-
-
3.10.1.2 Pseudomonas sp. K
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.10.1.2
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.10.1.2 160
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
3.10.1.2 -20°C, 0.04 M Tris-HCl buffer, pH 7.2, 0.2% gelatin, 3 months, 10% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.10.1.2 N-(n-amyl)sulfamate + H2O
-
 Pseudomonas sp. n-amylamine + sulfate
-
 ?
3.10.1.2 N-(n-amyl)sulfamate + H2O
-
 Pseudomonas sp. K n-amylamine + sulfate
-
 ?
3.10.1.2 N-(n-hexyl)sulfamate + H2O
-
 Pseudomonas sp. n-hexylamine + sulfate
-
 ?
3.10.1.2 N-(n-hexyl)sulfamate + H2O
-
 Pseudomonas sp. K n-hexylamine + sulfate
-
 ?
3.10.1.2 N-(n-octyl)sulfamate + H2O
-
 Pseudomonas sp. n-octylamine + sulfate
-
 ?
3.10.1.2 N-(n-octyl)sulfamate + H2O
-
 Pseudomonas sp. K n-octylamine + sulfate
-
 ?
3.10.1.2 N-(n-propyl)sulfamate + H2O
-
 Pseudomonas sp. n-propylamine + sulfate
-
 ?
3.10.1.2 N-(n-propyl)sulfamate + H2O
-
 Pseudomonas sp. K n-propylamine + sulfate
-
 ?
3.10.1.2 N-cyclohexylsulfamate + H2O
-
 Pseudomonas sp. cyclohexylamine + sulfate
-
 ?
3.10.1.2 N-cyclohexylsulfamate + H2O
-
 Pseudomonas sp. K cyclohexylamine + sulfate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.10.1.2 50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.10.1.2 50
-
 2 h without loss of activity Pseudomonas sp.
3.10.1.2 60
-
 40% loss of activity after 20 min Pseudomonas sp.
3.10.1.2 70
-
 completely inactivated after 5 min Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.10.1.2 6.5 6.7
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.10.1.2 5.9 7.5
-
 Pseudomonas sp.