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Literature summary extracted from

  • Guerrero, M.G.; Vega, J.M.; Losada, M.
    The assimilatory nitrate-reducing system and its regulation (1981), Annu. Rev. Plant Physiol., 32, 169-204.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.7.2 Cyanate stimulates Acinetobacter calcoaceticus
1.7.7.2 Cyanate stimulates Azotobacter chroococcum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.7.1 CO enzyme from higher plants and algae [Chlorella] fusca
1.7.7.1 CO enzyme from higher plants and algae Cucurbita pepo
1.7.7.1 CO enzyme from higher plants and algae Neopyropia yezoensis
1.7.7.1 CO enzyme from higher plants and algae Spinacia oleracea
1.7.7.1 cyanide
-
 [Chlorella] fusca
1.7.7.1 cyanide
-
 Cucurbita pepo
1.7.7.1 cyanide
-
 Neopyropia yezoensis
1.7.7.1 cyanide
-
 Spinacia oleracea
1.7.7.1 Mersalyl
-
 Cucurbita pepo
1.7.7.1 p-hydroxymercuribenzoate enzyme from higher plants and eukaryotic algae inhibited, cyanobacterial enzyme not [Chlorella] fusca
1.7.7.1 p-hydroxymercuribenzoate enzyme from higher plants and eukaryotic algae inhibited, cyanobacterial enzyme not Cucurbita pepo
1.7.7.1 p-hydroxymercuribenzoate enzyme from higher plants and eukaryotic algae inhibited, cyanobacterial enzyme not Neopyropia yezoensis
1.7.7.1 p-hydroxymercuribenzoate enzyme from higher plants and eukaryotic algae inhibited, cyanobacterial enzyme not Spinacia oleracea
1.7.7.2 cyanide
-
 Acinetobacter calcoaceticus
1.7.7.2 cyanide
-
 Azotobacter chroococcum
1.7.7.2 cyanide powerful inhibitor Synechococcus elongatus PCC 7942 = FACHB-805
1.7.7.2 p-hydroxymercuribenzoate
-
 Acinetobacter calcoaceticus
1.7.7.2 p-hydroxymercuribenzoate
-
 Azotobacter chroococcum
1.7.7.2 p-hydroxymercuribenzoate powerful inhibitor Synechococcus elongatus PCC 7942 = FACHB-805

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.2 additional information
-
 additional information
-
 Rhodotorula glutinis
1.7.1.2 additional information
-
 additional information
-
 Ankistrodesmus braunii
1.7.7.1 additional information
-
 additional information
-
 Spinacia oleracea
1.7.7.1 additional information
-
 additional information
-
 [Chlorella] fusca
1.7.7.1 additional information
-
 additional information
-
 Cucurbita pepo
1.7.7.1 additional information
-
 additional information
-
 Neopyropia yezoensis
1.7.7.2 0.013
-
 reduced ferredoxin
-
 Synechococcus elongatus PCC 7942 = FACHB-805
1.7.7.2 0.69
-
 nitrate
-
 Synechococcus elongatus PCC 7942 = FACHB-805

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.7.1 particle-bound chlorophyll-containing subcellular particle Synechococcus elongatus PCC 7942 = FACHB-805
-
-
1.7.7.1 soluble exception: Anacystis Synechococcus elongatus PCC 7942 = FACHB-805
-
-
1.7.7.2 membrane tightly bound to chlorophyll-containing membrane fractions Synechococcus elongatus PCC 7942 = FACHB-805 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.7.1 Iron
-
 [Chlorella] fusca
1.7.7.1 Iron
-
 Cucurbita pepo
1.7.7.1 Iron
-
 Neopyropia yezoensis
1.7.7.1 Iron enzyme contains 1 tetranuclear center (4Fe-4S) and 1 siroheme, minimum of 5 Fe atoms per active enzyme molecule Spinacia oleracea
1.7.7.2 Molybdenum molybdoprotein Synechococcus elongatus PCC 7942 = FACHB-805
1.7.7.2 Molybdenum molybdoprotein Acinetobacter calcoaceticus
1.7.7.2 Molybdenum molybdoprotein Rhodobacter capsulatus
1.7.7.2 Molybdenum molybdoprotein Azotobacter chroococcum
1.7.7.2 Molybdenum enzyme contains one molybdenum atom Rhodobacter capsulatus
1.7.7.2 Molybdenum essential role in catalytic activity Synechococcus elongatus PCC 7942 = FACHB-805

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.1 60000 63000 gel filtration, sedimentation equilibrium Spinacia oleracea
1.7.7.1 63000
-
-
 Spinacia oleracea
1.7.7.1 63000
-
-
 [Chlorella] fusca
1.7.7.1 63000
-
-
 Cucurbita pepo
1.7.7.1 63000
-
-
 Neopyropia yezoensis
1.7.7.2 75000
-
-
 Synechococcus elongatus PCC 7942 = FACHB-805
1.7.7.2 85000
-
 2 * 85000 Rhodobacter capsulatus
1.7.7.2 100000
-
 about Acinetobacter calcoaceticus
1.7.7.2 100000
-
 about Azotobacter chroococcum
1.7.7.2 185000
-
-
 Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.7.1 nitrite + reduced ferredoxin Synechococcus elongatus PCC 7942 = FACHB-805
-
 ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin Spinacia oleracea
-
 ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin [Chlorella] fusca
-
 ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin Cucurbita pepo
-
 ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin Neopyropia yezoensis
-
 ammonia + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin Synechococcus elongatus PCC 7942 = FACHB-805 assimilatory nitrate reduction nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin Anabaena cylindrica assimilatory nitrate reduction nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin Desmonostoc muscorum assimilatory nitrate reduction nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin Synechococcus elongatus PCC 7942 = FACHB-805 reduced ferredoxin as physiological electron donor nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin Anabaena cylindrica reduced ferredoxin as physiological electron donor nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin Desmonostoc muscorum reduced ferredoxin as physiological electron donor nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced flavodoxin Synechococcus elongatus PCC 7942 = FACHB-805 flavodoxin can physiologically substitute for ferredoxin under conditions of iron starvation nitrite + oxidized flavodoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.2 Ankistrodesmus braunii
-
-
-
1.7.1.2 Rhodotorula glutinis
-
-
-
1.7.7.1 Cucurbita pepo
-
-
-
1.7.7.1 Neopyropia yezoensis
-
-
-
1.7.7.1 Spinacia oleracea
-
-
-
1.7.7.1 Synechococcus elongatus PCC 7942 = FACHB-805
-
-
-
1.7.7.1 [Chlorella] fusca
-
-
-
1.7.7.2 Acinetobacter calcoaceticus
-
-
-
1.7.7.2 Anabaena cylindrica
-
-
-
1.7.7.2 Azotobacter chroococcum
-
-
-
1.7.7.2 Desmonostoc muscorum
-
-
-
1.7.7.2 Rhodobacter capsulatus
-
-
-
1.7.7.2 Synechococcus elongatus PCC 7942 = FACHB-805
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.2
-
 Synechococcus elongatus PCC 7942 = FACHB-805
1.7.7.2
-
 Rhodobacter capsulatus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.7.7.1 NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ mechanism Synechococcus elongatus PCC 7942 = FACHB-805
a
1.7.7.1 NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ mechanism Spinacia oleracea
a
1.7.7.1 NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ mechanism [Chlorella] fusca
a
1.7.7.1 NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ mechanism Cucurbita pepo
a
1.7.7.1 NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ mechanism Neopyropia yezoensis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.7.2 additional information
-
-
 Synechococcus elongatus PCC 7942 = FACHB-805
1.7.7.2 additional information
-
-
 Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.2 NAD(P)H + nitrate
-
 Rhodotorula glutinis NAD(P)+ + nitrite + H2O
-
 ?
1.7.1.2 NAD(P)H + nitrate
-
 Ankistrodesmus braunii NAD(P)+ + nitrite + H2O
-
 ?
1.7.7.1 nitrite + reduced ferredoxin
-
 Synechococcus elongatus PCC 7942 = FACHB-805 ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin
-
 Spinacia oleracea ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin
-
 [Chlorella] fusca ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin
-
 Cucurbita pepo ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin
-
 Neopyropia yezoensis ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donor: methyl viologen Spinacia oleracea ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donor: methyl viologen [Chlorella] fusca ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donor: methyl viologen Cucurbita pepo ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donor: methyl viologen Neopyropia yezoensis ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donors: flavodoxin Spinacia oleracea ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donors: flavodoxin [Chlorella] fusca ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donors: flavodoxin Cucurbita pepo ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donors: flavodoxin Neopyropia yezoensis ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate Spinacia oleracea ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate [Chlorella] fusca ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate Cucurbita pepo ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate Neopyropia yezoensis ammonia + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin
-
 Acinetobacter calcoaceticus nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin
-
 Rhodobacter capsulatus nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin
-
 Azotobacter chroococcum nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin enzyme can not accept electrons directly from NAD(P)H, it is dependent on reduced ferredoxin as physiological electron donor Synechococcus elongatus PCC 7942 = FACHB-805 nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin enzyme can not accept electrons directly from NAD(P)H, it is dependent on reduced ferredoxin as physiological electron donor Anabaena cylindrica nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin enzyme can not accept electrons directly from NAD(P)H, it is dependent on reduced ferredoxin as physiological electron donor Desmonostoc muscorum nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin assimilatory nitrate reduction Synechococcus elongatus PCC 7942 = FACHB-805 nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin assimilatory nitrate reduction Anabaena cylindrica nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin assimilatory nitrate reduction Desmonostoc muscorum nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin reduced ferredoxin as physiological electron donor Synechococcus elongatus PCC 7942 = FACHB-805 nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin reduced ferredoxin as physiological electron donor Anabaena cylindrica nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin reduced ferredoxin as physiological electron donor Desmonostoc muscorum nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced flavodoxin flavodoxin can physiologically substitute for ferredoxin under conditions of iron starvation Synechococcus elongatus PCC 7942 = FACHB-805 nitrite + oxidized flavodoxin + H2O
-
 ?
1.7.7.2 nitrate + reduced methyl viologen enzyme uses also reduced methyl viologen as reductant Synechococcus elongatus PCC 7942 = FACHB-805 nitrite + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.7.1 monomer
-
 Spinacia oleracea
1.7.7.1 monomer
-
 [Chlorella] fusca
1.7.7.2 dimer 2 * 85000 Rhodobacter capsulatus
1.7.7.2 monomer 1 * 75000 Synechococcus elongatus PCC 7942 = FACHB-805

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.7.1 10
-
 nitrite
-
 Neopyropia yezoensis
1.7.7.1 54
-
 nitrite
-
 [Chlorella] fusca
1.7.7.1 89
-
 nitrite
-
 Cucurbita pepo
1.7.7.1 110
-
 nitrite
-
 Spinacia oleracea
1.7.7.2 1090
-
 nitrate dithionite-reduced methyl viologen as electron donor Synechococcus elongatus PCC 7942 = FACHB-805

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.2 7.5
-
-
 Rhodotorula glutinis
1.7.1.2 7.5
-
-
 Ankistrodesmus braunii
1.7.7.1 7.5
-
-
 Spinacia oleracea
1.7.7.1 7.5
-
-
 [Chlorella] fusca
1.7.7.1 7.5
-
-
 Cucurbita pepo
1.7.7.1 7.5
-
-
 Neopyropia yezoensis
1.7.7.2 10.5
-
 dithionite-reduced methyl viologen as electron donor Synechococcus elongatus PCC 7942 = FACHB-805

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.2 cytochrome b557 1 mol per mol of enzyme Rhodotorula glutinis
1.7.1.2 cytochrome b557 4 mol per mol of enzyme Ankistrodesmus braunii
1.7.7.1 siroheme prosthetic group Spinacia oleracea
1.7.7.1 siroheme 1 mol siroheme per mol of enzyme Spinacia oleracea
1.7.7.2 heme c enzyme contains 1.5 heme molecules of the c-type Rhodobacter capsulatus
1.7.7.2 additional information no presence of flavin, heme or cytochrome Synechococcus elongatus PCC 7942 = FACHB-805