BRENDA - Enzyme Database

Purification and characterization of glutamyl-tRNA synthetase. An enzyme involved in chlorophyll biosynthesis

Chang, T.E.; Wegmann, B.; Wang, W.; Plant Physiol. 93, 1641-1649 (1990)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.1.1.17
heme
-
Chlamydomonas reinhardtii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
32500
-
2 * 32500, SDS-PAGE
Chlamydomonas reinhardtii
6.1.1.17
60000
-
HPLC gel filtration
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
Chlamydomonas reinhardtii
involved in synthesis of 5-aminolevulinate (a committed and regulated precursor in the chlorophyll biosynthetic pathway)
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Chlamydomonas reinhardtii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.17
-
Chlamydomonas reinhardtii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.1.1.17
additional information
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
208
Chlamydomonas reinhardtii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
208
Chlamydomonas reinhardtii
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
involved in synthesis of 5-aminolevulinate (a committed and regulated precursor in the chlorophyll biosynthetic pathway)
208
Chlamydomonas reinhardtii
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
6.1.1.17
dimer
2 * 32500, SDS-PAGE
Chlamydomonas reinhardtii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.1.1.17
heme
-
Chlamydomonas reinhardtii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
32500
-
2 * 32500, SDS-PAGE
Chlamydomonas reinhardtii
6.1.1.17
60000
-
HPLC gel filtration
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
Chlamydomonas reinhardtii
involved in synthesis of 5-aminolevulinate (a committed and regulated precursor in the chlorophyll biosynthetic pathway)
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
-
Chlamydomonas reinhardtii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.1.1.17
additional information
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
208
Chlamydomonas reinhardtii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
208
Chlamydomonas reinhardtii
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
involved in synthesis of 5-aminolevulinate (a committed and regulated precursor in the chlorophyll biosynthetic pathway)
208
Chlamydomonas reinhardtii
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.1.1.17
dimer
2 * 32500, SDS-PAGE
Chlamydomonas reinhardtii