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Literature summary extracted from

  • Magee, P.T.; Snell, E.E.
    The bacterial degradation of pantothenic acid. IV. Enzymatic conversion of aldopantoate to alpha-ketoisovalerate (1966), Biochemistry, 5, 409-416.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.1.33 aldopantoate stabilizes against heat Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.33 (R)-4-dehydropantoate
-
Pseudomonas sp.
1.2.1.33 NAD+ above 0.3 mM Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.84 0.15 0.2 3,3-dimethyl-D-malate
-
Pseudomonas sp.
1.1.1.84 0.27
-
NAD+
-
Pseudomonas sp.
1.1.1.84 0.33
-
3-(n-Propyl)malate
-
Pseudomonas sp.
1.1.1.84 8.9 11 D-malate
-
Pseudomonas sp.
1.2.1.33 0.07
-
NAD+
-
Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.84 Co2+ Mn2+ or Co2+ required Pseudomonas sp.
1.1.1.84 K+ K+ or NH4+ required Pseudomonas sp.
1.1.1.84 Mn2+ Mn2+ or Co2+ required Pseudomonas sp.
1.1.1.84 NH4+ K+ or NH4+ required Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.84 (R)-3,3-dimethylmalate + NAD+ Pseudomonas sp.
-
3-methyl-2-oxobutanoate + CO2 + NADH
-
r
1.1.1.84 (R)-3,3-dimethylmalate + NAD+ Pseudomonas sp. P-2
-
3-methyl-2-oxobutanoate + CO2 + NADH
-
r
1.2.1.33 (R)-4-dehydropantoate + NAD+ Pseudomonas sp. enzyme required for degradation of pantothenate to alpha-ketoisovalerate (R)-3,3-dimethylmalate + NADH
-
?
1.2.1.33 (R)-4-dehydropantoate + NAD+ Pseudomonas sp. P-2 enzyme required for degradation of pantothenate to alpha-ketoisovalerate (R)-3,3-dimethylmalate + NADH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.84 Pseudomonas sp.
-
P-2
-
1.1.1.84 Pseudomonas sp. P-2
-
P-2
-
1.2.1.33 Pseudomonas sp.
-
P-2
-
1.2.1.33 Pseudomonas sp. P-2
-
P-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.84
-
Pseudomonas sp.
1.2.1.33 P-2, partial Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.84 4.1
-
-
Pseudomonas sp.
1.2.1.33 0.4
-
-
Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.84 50 days, 0°C Pseudomonas sp.
1.2.1.33 0°C, 0.1 M potassium phosphate pH 7.5, 0.015 M mercaptoethanol, 3 days Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.84 (R)-3,3-dimethylmalate + NAD+
-
Pseudomonas sp. 3-methyl-2-oxobutanoate + CO2 + NADH
-
r
1.1.1.84 (R)-3,3-dimethylmalate + NAD+
-
Pseudomonas sp. P-2 3-methyl-2-oxobutanoate + CO2 + NADH
-
r
1.1.1.84 3-(n-propyl)malate + NAD+
-
Pseudomonas sp. 2-oxopentanoate + CO2 + NADH
-
?
1.1.1.84 3-(n-propyl)malate + NAD+
-
Pseudomonas sp. P-2 2-oxopentanoate + CO2 + NADH
-
?
1.1.1.84 D-malate + NAD+
-
Pseudomonas sp. pyruvate + CO2 + NADH
-
?
1.1.1.84 D-malate + NAD+
-
Pseudomonas sp. P-2 pyruvate + CO2 + NADH
-
?
1.2.1.33 (R)-4-dehydropantoate + NAD+ enzyme required for degradation of pantothenate to alpha-ketoisovalerate Pseudomonas sp. (R)-3,3-dimethylmalate + NADH
-
?
1.2.1.33 (R)-4-dehydropantoate + NAD+ enzyme required for degradation of pantothenate to alpha-ketoisovalerate Pseudomonas sp. P-2 (R)-3,3-dimethylmalate + NADH
-
?
1.2.1.33 (R)-4-dehydropantoate + NAD+ + H2O
-
Pseudomonas sp. (R)-3,3-dimethylmalate + NADH
-
?
1.2.1.33 (R)-4-dehydropantoate + NAD+ + H2O
-
Pseudomonas sp. P-2 (R)-3,3-dimethylmalate + NADH
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.84 60
-
5 min, 40% loss of activity Pseudomonas sp.
1.2.1.33 50
-
4 min in presence of aldopantoate: no loss of activity, 1 min in absence of aldopantoate, complete loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.84 8 8.5 substrates 3,3-dimethylmalate + NAD+ Pseudomonas sp.
1.2.1.33 9
-
or higher, activity increases from pH 7.0 to 9.5 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.33 7 9.5 activity increases from pH 7.0 to 9.5 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.84 NAD+
-
Pseudomonas sp.
1.2.1.33 additional information no diffusible cofactors found Pseudomonas sp.