EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.84 | 34000 | - |
4 * 34000, SDS-PAGE | Pseudomonas fluorescens |
1.1.1.84 | 136000 | - |
ultracentrifugation | Pseudomonas fluorescens |
1.1.1.84 | 142000 | - |
gel filtration | Pseudomonas fluorescens |
1.1.1.106 | 24000 | - |
4 * 24000, SDS-PAGE | Pseudomonas fluorescens |
1.1.1.106 | 80000 | - |
ultracentrifugation | Pseudomonas fluorescens |
1.1.1.106 | 86000 | - |
gel filtration | Pseudomonas fluorescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.84 | (R)-3,3-dimethylmalate + NAD+ | Pseudomonas fluorescens | - |
3-methyl-2-oxobutanoate + CO2 + NADH | - |
? | |
1.1.1.84 | (R)-3,3-dimethylmalate + NAD+ | Pseudomonas fluorescens UK1 | - |
3-methyl-2-oxobutanoate + CO2 + NADH | - |
? | |
1.1.1.106 | (R)-pantoate + NAD+ | Pseudomonas fluorescens | - |
(R)-4-dehydropantoate + NADH | - |
? | |
1.1.1.106 | (R)-pantoate + NAD+ | Pseudomonas fluorescens UK1 | - |
(R)-4-dehydropantoate + NADH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.84 | Pseudomonas fluorescens | - |
- |
- |
1.1.1.106 | Pseudomonas fluorescens | - |
UK1 | - |
1.1.1.106 | Pseudomonas fluorescens UK1 | - |
UK1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.84 | - |
Pseudomonas fluorescens |
1.1.1.106 | ammonium sulfate, 50°C, gel filtration, hydroxyapatite, 60°C, gel filtration | Pseudomonas fluorescens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.106 | 19.34 | - |
- |
Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.84 | (R)-3,3-dimethylmalate + NAD+ | - |
Pseudomonas fluorescens | 3-methyl-2-oxobutanoate + CO2 + NADH | - |
? | |
1.1.1.84 | (R)-3,3-dimethylmalate + NAD+ | - |
Pseudomonas fluorescens UK1 | 3-methyl-2-oxobutanoate + CO2 + NADH | - |
? | |
1.1.1.106 | (R)-pantoate + NAD+ | - |
Pseudomonas fluorescens | (R)-4-dehydropantoate + NADH | - |
? | |
1.1.1.106 | (R)-pantoate + NAD+ | - |
Pseudomonas fluorescens UK1 | (R)-4-dehydropantoate + NADH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.84 | tetramer | 4 * 34000, SDS-PAGE | Pseudomonas fluorescens |
1.1.1.106 | tetramer | 4 * 24000, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.84 | NAD+ | - |
Pseudomonas fluorescens | |
1.1.1.106 | NAD+ | - |
Pseudomonas fluorescens |