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Literature summary extracted from

  • Murata, K.; Fukuda, Y.; Simosaka, M.; Watanabe, K.; Saikusa, T.; Kimura, A.
    Metabolism of 2-oxoaldehyde in yeasts. Purification and characterization of NADPH-dependent methylglyoxal-reducing enzyme from Saccharomyces cerevisiae (1985), Eur. J. Biochem., 151, 631-636.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.283 2-mercaptoethanol activates Saccharomyces cerevisiae
1.1.1.283 dithiothreitol activates Saccharomyces cerevisiae
1.1.1.283 glutathione activates Saccharomyces cerevisiae
1.1.1.283 L-cysteine activates Saccharomyces cerevisiae
1.1.1.283 Triton X-100 activates Saccharomyces cerevisiae
1.1.1.283 Tween 80 activates Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.283 Hg2+
-
Saccharomyces cerevisiae
1.1.1.283 iodoacetate
-
Saccharomyces cerevisiae
1.1.1.283 N-dodecanoylsarcosine
-
Saccharomyces cerevisiae
1.1.1.283 N-ethylmaleimide
-
Saccharomyces cerevisiae
1.1.1.283 NADP+
-
Saccharomyces cerevisiae
1.1.1.283 p-chloromercuribenzoate
-
Saccharomyces cerevisiae
1.1.1.283 sodium cholate
-
Saccharomyces cerevisiae
1.1.1.283 sodium dodecylsulfate
-
Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.283 0.0002
-
NADPH
-
Saccharomyces cerevisiae
1.1.1.283 1.54
-
Phenylglyoxal
-
Saccharomyces cerevisiae
1.1.1.283 5.88
-
methylglyoxal
-
Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.78 43000
-
1 * 43000, SDS-PAGE Saccharomyces cerevisiae
1.1.1.283 43000
-
gel filtration, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.283 methylglyoxal + NADPH Saccharomyces cerevisiae similar enzyme with NADPH requirement, no reaction with NAD+, NADH and NADP+ lactaldehyde + NADP+
-
ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.283 Saccharomyces cerevisiae
-
similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.283 glycoprotein 6.6% carbohydrate, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.283 similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.78 (R)-lactaldehyde + NAD+ = 2-oxopropanal + NADH + H+ similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.283 8.38
-
-
Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.1.1.283 -20°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.283 glyoxal + NADPH
-
Saccharomyces cerevisiae glycolaldehyde + NADP+
-
ir
1.1.1.283 methylglyoxal + NADPH similar enzyme with NADPH requirement, no reaction with NAD+, NADH and NADP+ Saccharomyces cerevisiae lactaldehyde + NADP+
-
ir
1.1.1.283 methylglyoxal + NADPH + H+
-
Saccharomyces cerevisiae L-lactaldehyde + NADP+
-
r
1.1.1.283 additional information NADPH required Saccharomyces cerevisiae ?
-
?
1.1.1.283 phenylglyoxal + NADPH
-
Saccharomyces cerevisiae hydroxyphenylacetaldehyde + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.78 monomer 1 * 43000, SDS-PAGE Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.78 25
-
stable below Saccharomyces cerevisiae
1.1.1.78 39
-
3 min, 50% loss of activity Saccharomyces cerevisiae
1.1.1.78 44
-
80 s, 50% loss of activity Saccharomyces cerevisiae
1.1.1.78 50
-
40 s, 50% loss of activity Saccharomyces cerevisiae
1.1.1.78 60
-
35 s, 50% loss of activity Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.78 7
-
similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.78 6 8 pH 6.0 and pH 8.0: about 50% of activity maximum Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.78 4.5
-
1 min, 40°C, 50% loss of activity Saccharomyces cerevisiae
1.1.1.78 6
-
1 min, 40°C, stable Saccharomyces cerevisiae
1.1.1.78 8
-
1 min, 40°C, stable Saccharomyces cerevisiae
1.1.1.78 11
-
1 min, 40°C, 50% loss of activity 4 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.78 NADPH NADPH required Saccharomyces cerevisiae