Literature summary extracted from
Cane, D.E.; Hsiung, Y.; Cornish, J.A.; Robinson, J.K.; Spenser, I.D.
Biosynthesis of vitamin B6: the oxidation of 4-(phosphohydroxy)-L-threonine by PdxA (1998), J. Am. Chem. Soc., 120, 1936-1937.
No PubMed abstract available
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.1.1.262 |
overexpression in Escherichia coli |
Escherichia coli |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.1.1.262 |
4-(phosphohydroxy)-L-threonine + NAD+ |
Escherichia coli |
involved in pyridoxal 5'-phosphate formation |
2-amino-3-oxo-4-phosphohydroxybutyrate + NADH |
undergoes decarboxylation either catalysed by the enzyme or after release, further formation of pyridoxal 5'-phosphate catalysed by PdxJ protein |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.1.1.262 |
Escherichia coli |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.1.1.262 |
to homogeneity, recombinant enzyme |
Escherichia coli |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.1.1.262 |
additional information |
pdxA gene product |
Escherichia coli |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.1.1.262 |
4-(phosphohydroxy)-L-threonine + NAD+ |
involved in pyridoxal 5'-phosphate formation |
Escherichia coli |
2-amino-3-oxo-4-phosphohydroxybutyrate + NADH |
undergoes decarboxylation either catalysed by the enzyme or after release, further formation of pyridoxal 5'-phosphate catalysed by PdxJ protein |
r |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.1.1.262 |
monomer |
gel filtration, native PAGE, SDS-PAGE |
Escherichia coli |