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Literature summary extracted from

  • Cane, D.E.; Hsiung, Y.; Cornish, J.A.; Robinson, J.K.; Spenser, I.D.
    Biosynthesis of vitamin B6: the oxidation of 4-(phosphohydroxy)-L-threonine by PdxA (1998), J. Am. Chem. Soc., 120, 1936-1937.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.262 overexpression in Escherichia coli Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.262 4-(phosphohydroxy)-L-threonine + NAD+ Escherichia coli involved in pyridoxal 5'-phosphate formation 2-amino-3-oxo-4-phosphohydroxybutyrate + NADH undergoes decarboxylation either catalysed by the enzyme or after release, further formation of pyridoxal 5'-phosphate catalysed by PdxJ protein r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.262 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.262 to homogeneity, recombinant enzyme Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.262 additional information pdxA gene product Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.262 4-(phosphohydroxy)-L-threonine + NAD+ involved in pyridoxal 5'-phosphate formation Escherichia coli 2-amino-3-oxo-4-phosphohydroxybutyrate + NADH undergoes decarboxylation either catalysed by the enzyme or after release, further formation of pyridoxal 5'-phosphate catalysed by PdxJ protein r

Subunits

EC Number Subunits Comment Organism
1.1.1.262 monomer gel filtration, native PAGE, SDS-PAGE Escherichia coli