Any feedback?
Literature summary extracted from
- Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: Characterization of the biosynthetic enzyme for the enantiomeric glycerophhosphate backbone of ether polar lipids of Archaea (1997), J. Biochem., 122, 572-576.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.261 | Co2+ | slight inhibition at 1 mM | Methanothermobacter thermautotrophicus |  |
| 1.1.1.261 | Cu2+ | slight inhibition at 1 mM | Methanothermobacter thermautotrophicus | |
| 1.1.1.261 | DTT | 45% inhibition at 1 mM | Methanothermobacter thermautotrophicus | |
| 1.1.1.261 | Ni2+ | slight inhibition at 1 mM | Methanothermobacter thermautotrophicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.261 | 0.025 | - |
NADPH | dihydroxyacetone phosphate reduction | Methanothermobacter thermautotrophicus | |
| 1.1.1.261 | 0.127 | - |
NAD+ | glycerol-1-phosphate oxidation | Methanothermobacter thermautotrophicus | |
| 1.1.1.261 | 0.129 | - |
NADH | dihydroxyacetone phosphate reduction | Methanothermobacter thermautotrophicus | |
| 1.1.1.261 | 0.27 | - |
NADP+ | glycerol-1-phosphate oxidation | Methanothermobacter thermautotrophicus | |
| 1.1.1.261 | 0.58 | - |
dihydroxyacetone phosphate | in the presence of NADPH | Methanothermobacter thermautotrophicus | |
| 1.1.1.261 | 2.17 | - |
dihydroxyacetone phosphate | - |
Methanothermobacter thermautotrophicus | |
| 1.1.1.261 | 4.8 | - |
glycerol-1-phosphate | in the presence of NADPH | Methanothermobacter thermautotrophicus | |
| 1.1.1.261 | 16.3 | - |
glycerol-1-phosphate | - |
Methanothermobacter thermautotrophicus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.261 | K+ | maximum activity at 60-80 mM | Methanothermobacter thermautotrophicus | |
| 1.1.1.261 | Na+ | maximum activity 20-25 mM | Methanothermobacter thermautotrophicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.261 | 38000 | - |
8 * 38000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 1.1.1.261 | 302000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.261 | dihydroxyacetone phosphate + NAD(P)H | Methanothermobacter thermautotrophicus | formation of glycerol-1-phosphate is the natural direction of the reaction | sn-glycerol-1-phosphate + NAD(P)+ | - |
r | |
| 1.1.1.261 | sn-glycerol-1-phosphate + NAD+ | Methanothermobacter thermautotrophicus | - |
dihydroxyacetone phosphate + NADH | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.261 | Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.261 | to homogeneity, 7step chromatography | Methanothermobacter thermautotrophicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.261 | cell culture | - |
Methanothermobacter thermautotrophicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.261 | 314 | - |
- |
Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.261 | dihydroxyacetone phosphate + NAD(P)H | formation of glycerol-1-phosphate is the natural direction of the reaction | Methanothermobacter thermautotrophicus | sn-glycerol-1-phosphate + NAD(P)+ | - |
r | |
| 1.1.1.261 | dihydroxyacetonephosphate + NAD(P)H | - |
Methanothermobacter thermautotrophicus | glycerol-1-phosphate + NAD(P)+ | - |
? | |
| 1.1.1.261 | sn-glycerol-1-phosphate + NAD+ | - |
Methanothermobacter thermautotrophicus | dihydroxyacetone phosphate + NADH | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.261 | octamer | 8 * 38000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.261 | 75 | - |
- |
Methanothermobacter thermautotrophicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.261 | 51 | - |
temperature-induced conformational change | Methanothermobacter thermautotrophicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.261 | 6.6 | 7.4 | - |
Methanothermobacter thermautotrophicus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.261 | 6.6 | - |
decrease of activity below | Methanothermobacter thermautotrophicus |
| 1.1.1.261 | 7.4 | - |
decrease of activity above | Methanothermobacter thermautotrophicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.261 | NAD+ | - |
Methanothermobacter thermautotrophicus | |
| 1.1.1.261 | NADP+ | - |
Methanothermobacter thermautotrophicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
