Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Junker, F.; Kiewitz, R.; Cook, A.M.
    Characterization of the p-toluenesulfonate operon tsaMBCD and tsaR in Comamonas testosteroni T-2 (1997), J. Bacteriol., 179, 919-927.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.257 Fe2+ 2Fe-2S-Rieske center Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.257 51000
-
 2 * 51000, SDS-PAGE Comamonas testosteroni
1.1.1.257 102000
-
 gel filtration Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O Comamonas testosteroni
-
 4-sulfobenzoate + NADH
-
 ?
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O Comamonas testosteroni T-2
-
 4-sulfobenzoate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.257 Comamonas testosteroni
-
 within a cluster of a four-gene operon, that defines a regulatory unit RI, that is involved in toluenesulfonate degradation
-
1.1.1.257 Comamonas testosteroni T-2
-
 within a cluster of a four-gene operon, that defines a regulatory unit RI, that is involved in toluenesulfonate degradation
-
1.2.1.62 Comamonas testosteroni
-
-
-
1.2.1.62 Comamonas testosteroni T-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.257 to homogeneity, chromatography techniques Comamonas testosteroni
1.2.1.62 to homogeneity, 2step chromatography Comamonas testosteroni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.257 cell culture
-
 Comamonas testosteroni
-
1.2.1.62 cell culture
-
 Comamonas testosteroni
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.62 30
-
-
 Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.257 4-carboxybenzaldehyde + NADH involved in the degradation of toluenesulfonate and toluenecarboxylate Comamonas testosteroni 4-carboxybenzyl alcohol + NAD+
-
 r
1.1.1.257 4-carboxybenzaldehyde + NADH involved in the degradation of toluenesulfonate and toluenecarboxylate Comamonas testosteroni T-2 4-carboxybenzyl alcohol + NAD+
-
 r
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O
-
 Comamonas testosteroni 4-sulfobenzoate + NADH
-
 ?
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O
-
 Comamonas testosteroni T-2 4-sulfobenzoate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.257 dimer 2 * 51000, SDS-PAGE Comamonas testosteroni

Synonyms

EC Number Synonyms Comment Organism
1.1.1.257 sulfobenzyl alcohol dehydrogenase
-
 Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.257 NAD+
-
 Comamonas testosteroni
1.2.1.62 NAD+
-
 Comamonas testosteroni