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Literature summary extracted from

  • Junker, F.; Saller, E.; Schläfli Oppenberg, H.R.; Kroneck, P.M.H.; Leisinger, T.; Cook, A.M.
    Degradative pathways for p-toluenecarboxylate and p-toluenesulfonate and their multicomponent oxygenases in Comamonas testosteroni strains PSB-4 and T-2 (1996), Microbiology, 142, 2419-2427.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.8 Fe2+
-
 Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.8 50000
-
 dioxygenase A, SDS-PAGE Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O Comamonas testosteroni
-
 4-sulfobenzoate + NADH
-
 ?
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O Comamonas testosteroni PSB-4
-
 4-sulfobenzoate + NADH
-
 ?
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O Comamonas testosteroni T-2
-
 4-sulfobenzoate + NADH
-
 ?
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O Comamonas testosteroni TER-1:
-
 4-sulfobenzoate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.257 Comamonas testosteroni
-
-
-
1.1.1.257 Comamonas testosteroni
-
 strain PSB-4: loss of reductase component to utilize p-toluenesulfonate to sulfobenzoate , strain TER-1: not able to utilize p-tolunenesulfonate and sulfobenzoate due to deletion of genetic material
-
1.1.1.257 Comamonas testosteroni T-2
-
-
-
1.1.1.257 Comamonas testosteroni TER-1:
-
 strain PSB-4: loss of reductase component to utilize p-toluenesulfonate to sulfobenzoate , strain TER-1: not able to utilize p-tolunenesulfonate and sulfobenzoate due to deletion of genetic material
-
1.2.1.62 Comamonas testosteroni
-
-
-
1.2.1.62 Comamonas testosteroni T-2
-
-
-
1.14.12.8 Comamonas testosteroni
-
 T-2
-
1.14.12.8 Comamonas testosteroni
-
 PSB-4
-
1.14.12.8 Comamonas testosteroni PSB-4
-
 PSB-4
-
1.14.12.8 Comamonas testosteroni T-2
-
 T-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.8 purification of the oxygenase system Comamonas testosteroni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.257 cell culture
-
 Comamonas testosteroni
-
1.2.1.62 cell culture
-
 Comamonas testosteroni
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.8 0.03
-
 for enzyme system grown on sulfobenzoate Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.257 4-carboxybenzaldehyde + NADH involved in the degradation of toluenesulfonate and toluenecarboxylate Comamonas testosteroni 4-carboxybenzyl alcohol + NAD+
-
 r
1.1.1.257 4-carboxybenzaldehyde + NADH involved in the degradation of toluenesulfonate and toluenecarboxylate Comamonas testosteroni PSB-4 4-carboxybenzyl alcohol + NAD+
-
 r
1.1.1.257 4-carboxybenzaldehyde + NADH involved in the degradation of toluenesulfonate and toluenecarboxylate Comamonas testosteroni T-2 4-carboxybenzyl alcohol + NAD+
-
 r
1.1.1.257 4-carboxybenzaldehyde + NADH involved in the degradation of toluenesulfonate and toluenecarboxylate Comamonas testosteroni TER-1: 4-carboxybenzyl alcohol + NAD+
-
 r
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O
-
 Comamonas testosteroni 4-sulfobenzoate + NADH
-
 ?
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O
-
 Comamonas testosteroni PSB-4 4-sulfobenzoate + NADH
-
 ?
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O
-
 Comamonas testosteroni T-2 4-sulfobenzoate + NADH
-
 ?
1.2.1.62 4-formylbenzenesulfonate + NAD+ + H2O
-
 Comamonas testosteroni TER-1: 4-sulfobenzoate + NADH
-
 ?
1.14.12.8 4-sulfobenzoate + NADH + O2
-
 Comamonas testosteroni 3,4-dihydroxybenzoate + sulfite + NAD+
-
 ?
1.14.12.8 4-sulfobenzoate + NADH + O2
-
 Comamonas testosteroni PSB-4 3,4-dihydroxybenzoate + sulfite + NAD+
-
 ?
1.14.12.8 4-sulfobenzoate + NADH + O2
-
 Comamonas testosteroni T-2 3,4-dihydroxybenzoate + sulfite + NAD+
-
 ?
1.14.12.8 4-sulfobenzoate + NADH + O2
-
 Comamonas testosteroni TER-1: 3,4-dihydroxybenzoate + sulfite + NAD+
-
 ?
1.14.12.8 4-toluenesulfonate + NADH + O2 this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC) Comamonas testosteroni ?
-
 ?
1.14.12.8 4-toluenesulfonate + NADH + O2 this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC) Comamonas testosteroni PSB-4 ?
-
 ?
1.14.12.8 4-toluenesulfonate + NADH + O2 this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC) Comamonas testosteroni T-2 ?
-
 ?
1.14.12.8 4-toluenesulfonate + NADH + O2 this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC) Comamonas testosteroni TER-1: ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.257 NAD+
-
 Comamonas testosteroni
1.2.1.62 NAD+
-
 Comamonas testosteroni