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Literature summary extracted from
- Physico-chemical characterization of a recombinant cytoplasmic form of lysine:N6-hydroxylase (1993), Biochim. Biophys. Acta, 1203, 27-35.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.59 | Carbonylcyanide-m-chlorophenylhydrazone | - |
Escherichia coli |  |
| 1.14.13.59 | Carbonylcyanide-p-fluoromethoxyphenylhydrazone | - |
Escherichia coli | |
| 1.14.13.59 | Cinnamylidene | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.59 | 0.1 | - |
NADPH | recombinant enzyme form IucD398, with a deletion of 47 amino acids in the N-terminus | Escherichia coli | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.59 | cytoplasm | - |
Escherichia coli | 5737 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.59 | Escherichia coli | - |
recombinant enzyme form IucD398, with a deletion of 47 amino acids in the N-terminus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.59 | 0.168 | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.13.59 | 4°C, medium of ionic strength 0.25 or higher, recombinant enzyme form IacD398, stable for 1 month | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.59 | (S)-2-Aminoethyl-L-Cys + NADPH + O2 | - |
Escherichia coli | ? | - |
? | |
| 1.14.13.59 | L-Lys + NADH + O2 | with lower efficiency than NADPH, recombinant enzyme form IucD398, with a deletion of 47 amino acids in the N-terminus | Escherichia coli | ? | - |
? | |
| 1.14.13.59 | L-Lys + NADPH + O2 | - |
Escherichia coli | N6-Hydroxy-L-Lys + NADP+ + H2O | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.59 | FAD | Km: 0.0051 mM | Escherichia coli | |
| 1.14.13.59 | FAD | requires FAD | Escherichia coli | |
| 1.14.13.59 | NADH | recombinant enzyme form IucD398, with a deletion of 47 amino acids in the N-terminus | Escherichia coli | |
| 1.14.13.59 | NADPH | required | Escherichia coli | |
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