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Literature summary extracted from

  • Plattner, H.J.; Pfefferle, P.; Romaguera, A.; Waschutza, S.; Dieckman, H.
    Isolation and some properties of lysine N6-hydroxylase from Escherichia coli strain EN222 (1989), Biol. Met., 2, 1-5.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.59 DL-2,3-Diaminopropionic acid stimulates, but remains unchanged Escherichia coli
1.14.13.59 DL-2,6-diaminopimelic acid stimulates, but remains unchanged Escherichia coli
1.14.13.59 L-Orn stimulates, but remains unchanged Escherichia coli
1.14.13.59 N6-Acetyl-L-Lys stimulates, but remains unchanged Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.59 p-chloromercuribenzoate 0.01 mM, 62% inhibition, reversed by dithiothreitol Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.59 0.018
-
 NADH
-
 Escherichia coli
1.14.13.59 0.105
-
 L-Lys
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.59 soluble
-
 Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.59 50000
-
 4 * 50000, SDS-PAGE Escherichia coli
1.14.13.59 200000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.59 Escherichia coli
-
 containing the structural gene for the enzyme on a multicopy plasmid
-
1.14.13.59 Escherichia coli EN222
-
 containing the structural gene for the enzyme on a multicopy plasmid
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.59 0.189
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.59 (S)-2-Aminoethyl-L-Cys + NADPH + O2 i.e. L-aminoethylcysteine Escherichia coli ?
-
 ?
1.14.13.59 (S)-2-Aminoethyl-L-Cys + NADPH + O2 i.e. L-aminoethylcysteine Escherichia coli EN222 ?
-
 ?
1.14.13.59 1,5-Diaminopentane + NADPH + O2
-
 Escherichia coli ?
-
 ?
1.14.13.59 DL-4-Selenalysine + NADPH + O2
-
 Escherichia coli ?
-
 ?
1.14.13.59 DL-4-Selenalysine + NADPH + O2
-
 Escherichia coli EN222 ?
-
 ?
1.14.13.59 DL-Homocysteine + NADPH + O2
-
 Escherichia coli ?
-
 ?
1.14.13.59 DL-Homocysteine + NADPH + O2
-
 Escherichia coli EN222 ?
-
 ?
1.14.13.59 DL/DL-Allo-delta-hydroxylysine + NADPH + O2
-
 Escherichia coli ?
-
 ?
1.14.13.59 L-Lys + NADH + O2
-
 Escherichia coli ?
-
 ?
1.14.13.59 L-Lys + NADH + O2
-
 Escherichia coli EN222 ?
-
 ?
1.14.13.59 L-Lys + NADPH + O2 specific for NADPH Escherichia coli N6-Hydroxy-L-Lys + NADP+ + H2O
-
 ?
1.14.13.59 L-Lys + NADPH + O2 specific for NADPH Escherichia coli EN222 N6-Hydroxy-L-Lys + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.59 tetramer 4 * 50000, SDS-PAGE Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.59 FAD contains 0.79 mol FAD per mol of subunit Escherichia coli
1.14.13.59 NADPH required Escherichia coli