EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.47 | ascorbate | - |
Arthrobacter sp. | |
1.13.11.47 | Ca2+ | weak | Arthrobacter sp. | |
1.13.11.47 | Co2+ | weak | Arthrobacter sp. | |
1.13.11.47 | Cu2+ | strong | Arthrobacter sp. | |
1.13.11.47 | Cu2+ | - |
Pseudomonas putida | |
1.13.11.47 | ethylxanthate | - |
Arthrobacter sp. | |
1.13.11.47 | Fe2+ | strong | Arthrobacter sp. | |
1.13.11.47 | H2O2 | - |
Arthrobacter sp. | |
1.13.11.47 | H2O2 | - |
Pseudomonas putida | |
1.13.11.47 | iodoacetamide | - |
Arthrobacter sp. | |
1.13.11.47 | Mg2+ | weak | Arthrobacter sp. | |
1.13.11.47 | Mn2+ | weak | Arthrobacter sp. | |
1.13.11.47 | NEM | - |
Arthrobacter sp. | |
1.13.11.47 | Ni2+ | strong | Arthrobacter sp. | |
1.13.11.47 | p-hydroxymercuribenzoate | - |
Arthrobacter sp. | |
1.13.11.47 | Sodium azide | - |
Arthrobacter sp. | |
1.13.11.47 | Sodium dithionite | - |
Arthrobacter sp. | |
1.13.11.47 | Zn2+ | strong | Arthrobacter sp. | |
1.13.11.47 | Zn2+ | - |
Pseudomonas putida | |
1.13.11.48 | Ca2+ | 1 mM, weak inhibition | Arthrobacter sp. | |
1.13.11.48 | Co2+ | 1 mM, weak inhibition | Arthrobacter sp. | |
1.13.11.48 | Cu2+ | 1 mM, strong inhibition | Arthrobacter sp. | |
1.13.11.48 | Fe2+ | 1 mM, strong inhibition | Arthrobacter sp. | |
1.13.11.48 | Mg2+ | 1 mM, weak inhibition | Arthrobacter sp. | |
1.13.11.48 | Mn2+ | 1 mM, weak inhibition | Arthrobacter sp. | |
1.13.11.48 | Ni2+ | 1 mM, strong inhibition | Arthrobacter sp. | |
1.13.11.48 | Zn2+ | 1 mM, strong inhibition | Arthrobacter sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.47 | 0.024 | - |
1H-3-Hydroxy-4-oxoquinoline | - |
Pseudomonas putida | |
1.13.11.47 | 0.03 | - |
1H-3-Hydroxy-4-oxoquinaldine | - |
Arthrobacter sp. | |
1.13.11.48 | 0.03 | - |
1H-3-Hydroxy-4-oxoquinaldine | - |
Arthrobacter sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.47 | copper | contains 0.048 mol of Cu per mol of enzyme | Pseudomonas putida | |
1.13.11.47 | additional information | addition of metal ions in the absence and in the presence of the reductant ascorbate does not increase activity | Pseudomonas putida | |
1.13.11.47 | additional information | addition of metal ions in the absence and in the presence of the reductant ascorbate does not increase activity | Arthrobacter sp. | |
1.13.11.47 | Nickel | contains 0.038 mol of Ni per mol of enzyme | Pseudomonas putida | |
1.13.11.47 | Nickel | contains 0.02 mol of Ni per mol of enzyme | Arthrobacter sp. | |
1.13.11.47 | Zinc | contains 0.045 mol of zinc per mol of enzyme | Arthrobacter sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.47 | 30000 | - |
gel filtration | Pseudomonas putida |
1.13.11.47 | 32000 | - |
gel filtration | Arthrobacter sp. |
1.13.11.48 | 32000 | - |
gel filtration | Arthrobacter sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.47 | Arthrobacter sp. | - |
strain R³61a | - |
1.13.11.47 | Arthrobacter sp. Ru61a | - |
strain R³61a | - |
1.13.11.47 | Pseudomonas putida | - |
- |
- |
1.13.11.47 | Pseudomonas putida 33/1 | - |
- |
- |
1.13.11.48 | Arthrobacter sp. | - |
Ru61a | - |
1.13.11.48 | Arthrobacter sp. Ru61a | - |
Ru61a | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.47 | - |
Pseudomonas putida |
1.13.11.47 | - |
Arthrobacter sp. |
1.13.11.48 | - |
Arthrobacter sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.47 | culture condition:quinaldine-grown cell | - |
Arthrobacter sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.47 | 13.1 | - |
- |
Pseudomonas putida |
1.13.11.47 | 70.7 | - |
- |
Arthrobacter sp. |
1.13.11.48 | 70.7 | - |
- |
Arthrobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.47 | 1H-3-Hydroxy-4-oxoquinaldine + O2 | - |
Arthrobacter sp. | N-Acetylanthranilate + CO | - |
? | |
1.13.11.47 | 1H-3-Hydroxy-4-oxoquinaldine + O2 | - |
Arthrobacter sp. Ru61a | N-Acetylanthranilate + CO | - |
? | |
1.13.11.47 | 1H-3-Hydroxy-4-oxoquinoline + O2 | - |
Pseudomonas putida | N-Formylanthranilate + CO | - |
? | |
1.13.11.47 | 1H-3-Hydroxy-4-oxoquinoline + O2 | at 20% of the activity with 1H-3-Hydroxy-4-oxoquinaldine | Arthrobacter sp. | N-Formylanthranilate + CO | - |
? | |
1.13.11.47 | 1H-3-Hydroxy-4-oxoquinoline + O2 | - |
Pseudomonas putida 33/1 | N-Formylanthranilate + CO | - |
? | |
1.13.11.47 | 1H-3-Hydroxy-4-oxoquinoline + O2 | at 20% of the activity with 1H-3-Hydroxy-4-oxoquinaldine | Arthrobacter sp. Ru61a | N-Formylanthranilate + CO | - |
? | |
1.13.11.48 | 1H-3-hydroxy-4-oxoquinaldine + O2 | 1H-3-hydroxy-4-oxoquinaldine oxygenase | Arthrobacter sp. | N-acetylanthranilic acid + CO | - |
ir | |
1.13.11.48 | 1H-3-hydroxy-4-oxoquinaldine + O2 | 1H-3-hydroxy-4-oxoquinaldine oxygenase | Arthrobacter sp. Ru61a | N-acetylanthranilic acid + CO | - |
ir | |
1.13.11.48 | 1H-3-hydroxy-4-oxoquinoline + O2 | also 1H-3-hydroxy-4-oxoquinaldine oxygenase | Arthrobacter sp. | N-formylanthranilic acid + CO | - |
ir | |
1.13.11.48 | 1H-3-hydroxy-4-oxoquinoline + O2 | also 1H-3-hydroxy-4-oxoquinaldine oxygenase | Arthrobacter sp. Ru61a | N-formylanthranilic acid + CO | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.47 | monomer | 1 * 30000, SDS-PAGE | Pseudomonas putida |
1.13.11.47 | monomer | 1 * 32000, SDS-PAGE | Arthrobacter sp. |
1.13.11.48 | monomer | SDS-PAGE | Arthrobacter sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.47 | 25 | - |
- |
Pseudomonas putida |
1.13.11.47 | 30 | - |
- |
Arthrobacter sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.47 | 30 | - |
labile above | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.47 | 8 | - |
- |
Pseudomonas putida |
1.13.11.47 | 8 | - |
- |
Arthrobacter sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.47 | additional information | no spectral evidence for the presence of a chromophoric cofactor | Pseudomonas putida | |
1.13.11.47 | additional information | no spectral evidence for the presence of a chromophoric cofactor | Arthrobacter sp. | |
1.13.11.48 | additional information | no cofactors | Arthrobacter sp. |