EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.1.1.3 | additional information | pyridoxal phosphate-independent enzyme | Pediococcus pentosaceus | |
5.1.1.13 | additional information | - |
Enterococcus faecalis | |
5.1.1.13 | additional information | enzyme contains no bound pyridoxal phosphate | Streptococcus thermophilus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.1.1.3 | expression in Escherichia coli | Pediococcus pentosaceus |
5.1.1.13 | expression in Escherichia coli | Streptococcus thermophilus |
EC Number | General Stability | Organism |
---|---|---|
5.1.1.3 | irreversible inactivation upon storage in the absence of 10% glycerol, 1 mM DL-Glu, and 0.1% 2-mercaptoethanol | Pediococcus pentosaceus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.1.1.13 | 2-Nitro-5-thiocyanobenzoate | - |
Streptococcus thermophilus | |
5.1.1.13 | additional information | enzyme is not influenced by high concentrations of aspartate up to 500 mM; no inhibition by: hydroxylamine, sodium borohydride | Streptococcus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.1.3 | 15 | - |
D-Glu | - |
Pediococcus pentosaceus | |
5.1.1.13 | 8.7 | - |
D-Asp | - |
Streptococcus thermophilus | |
5.1.1.13 | 35 | - |
L-Asp | - |
Streptococcus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.1.1.3 | 29000 | - |
gel filtration | Pediococcus pentosaceus |
5.1.1.13 | 28000 | - |
2 * 28000, SDS-PAGE | Streptococcus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.1.3 | Limosilactobacillus fermentum | - |
- |
- |
5.1.1.3 | Pediococcus pentosaceus | - |
- |
- |
5.1.1.13 | Enterococcus faecalis | - |
strain ATCC 9790, strain IAM 10065, no activity in strain IAM 10067 | - |
5.1.1.13 | Streptococcus thermophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.1.3 | - |
Pediococcus pentosaceus |
5.1.1.13 | - |
Enterococcus faecalis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.1.1.13 | 19.9 | - |
- |
Streptococcus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.3 | D-Glu | - |
Limosilactobacillus fermentum | L-Glu | - |
? | |
5.1.1.3 | D-Glu | - |
Pediococcus pentosaceus | L-Glu | - |
? | |
5.1.1.13 | L-Asp | - |
Enterococcus faecalis | D-Asp | - |
? | |
5.1.1.13 | L-Asp | - |
Streptococcus thermophilus | D-Asp | - |
? | |
5.1.1.13 | L-Cysteate | - |
Streptococcus thermophilus | D-Cysteate | - |
? | |
5.1.1.13 | L-Cysteine sulfinate | - |
Streptococcus thermophilus | D-Cysteine sulfinate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.1.1.3 | monomer | 1 * 29000, SDS-PAGE | Pediococcus pentosaceus |
5.1.1.13 | dimer | 2 * 28000, SDS-PAGE | Streptococcus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.1.3 | 8 | - |
- |
Pediococcus pentosaceus |