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Literature summary extracted from

  • Soda, K.
    PLP-dependent and independent amino acid racemase (1991), Enzymes Depend. Pyridoxal Phosphate Other Carbonyl Comp. Cofactors (Proc. Int. Symp. Vitam. B6 Carbonyl Catal. , Meeting Date 1990, Fukui T. , ed. ) Pergamon, Oxford, 8, 29-34.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.1.3 additional information pyridoxal phosphate-independent enzyme Pediococcus pentosaceus
5.1.1.13 additional information
-
 Enterococcus faecalis
5.1.1.13 additional information enzyme contains no bound pyridoxal phosphate Streptococcus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.3 expression in Escherichia coli Pediococcus pentosaceus
5.1.1.13 expression in Escherichia coli Streptococcus thermophilus

General Stability

EC Number General Stability Organism
5.1.1.3 irreversible inactivation upon storage in the absence of 10% glycerol, 1 mM DL-Glu, and 0.1% 2-mercaptoethanol Pediococcus pentosaceus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.13 2-Nitro-5-thiocyanobenzoate
-
 Streptococcus thermophilus
5.1.1.13 additional information enzyme is not influenced by high concentrations of aspartate up to 500 mM; no inhibition by: hydroxylamine, sodium borohydride Streptococcus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.3 15
-
 D-Glu
-
 Pediococcus pentosaceus
5.1.1.13 8.7
-
 D-Asp
-
 Streptococcus thermophilus
5.1.1.13 35
-
 L-Asp
-
 Streptococcus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.3 29000
-
 gel filtration Pediococcus pentosaceus
5.1.1.13 28000
-
 2 * 28000, SDS-PAGE Streptococcus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.3 Limosilactobacillus fermentum
-
-
-
5.1.1.3 Pediococcus pentosaceus
-
-
-
5.1.1.13 Enterococcus faecalis
-
 strain ATCC 9790, strain IAM 10065, no activity in strain IAM 10067
-
5.1.1.13 Streptococcus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.3
-
 Pediococcus pentosaceus
5.1.1.13
-
 Enterococcus faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.13 19.9
-
-
 Streptococcus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.3 D-Glu
-
 Limosilactobacillus fermentum L-Glu
-
 ?
5.1.1.3 D-Glu
-
 Pediococcus pentosaceus L-Glu
-
 ?
5.1.1.13 L-Asp
-
 Enterococcus faecalis D-Asp
-
 ?
5.1.1.13 L-Asp
-
 Streptococcus thermophilus D-Asp
-
 ?
5.1.1.13 L-Cysteate
-
 Streptococcus thermophilus D-Cysteate
-
 ?
5.1.1.13 L-Cysteine sulfinate
-
 Streptococcus thermophilus D-Cysteine sulfinate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.3 monomer 1 * 29000, SDS-PAGE Pediococcus pentosaceus
5.1.1.13 dimer 2 * 28000, SDS-PAGE Streptococcus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.3 8
-
-
 Pediococcus pentosaceus