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Literature summary extracted from
- Purification and properties of a pyridoxal phosphate and coenzyme B12 dependent D-alpha-ornithine 5,4-aminomutase (1973), Biochemistry, 12, 2597-2604.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.3.5 | mercaptan | keeps essential labile sulfhydryl groups reduced and protects from oxygen | Acetoanaerobium sticklandii |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.4.3.5 | glycerol stabilizes during storage and purification | Acetoanaerobium sticklandii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.3.5 | 2,4-Diaminopentanoic acid | product inhibition above 0.7 mM | Acetoanaerobium sticklandii | |
| 5.4.3.5 | beta-Lys | - |
Acetoanaerobium sticklandii | |
| 5.4.3.5 | DL-alpha-Lys | - |
Acetoanaerobium sticklandii | |
| 5.4.3.5 | iodoacetate | - |
Acetoanaerobium sticklandii | |
| 5.4.3.5 | KCl | - |
Acetoanaerobium sticklandii | |
| 5.4.3.5 | L-alpha-Orn | - |
Acetoanaerobium sticklandii | |
| 5.4.3.5 | NEM | - |
Acetoanaerobium sticklandii | |
| 5.4.3.5 | PCMB | - |
Acetoanaerobium sticklandii | |
| 5.4.3.5 | potassium phosphate | - |
Acetoanaerobium sticklandii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.3.5 | 0.44 | - |
D-Orn | coupled assay with C4 dehydrogenase | Acetoanaerobium sticklandii | |
| 5.4.3.5 | 6.7 | - |
D-Orn | radiochemical assay | Acetoanaerobium sticklandii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.5 | 180000 | - |
gel electrophoresis, gel filtration, sucrose density gradient centrifugation | Acetoanaerobium sticklandii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.3.5 | D-Orn | Acetoanaerobium sticklandii | enzyme of ornithine fermentation | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.12 | Acetoanaerobium sticklandii | - |
the product of this reaction is not identical with D-ornithine, the literature reference is probably incorrect | - |
| 5.4.3.5 | Acetoanaerobium sticklandii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.3.5 | - |
Acetoanaerobium sticklandii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.5 | 4.38 | - |
- |
Acetoanaerobium sticklandii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.4.3.5 | -20°C, at a concentration of 0.7 mg/ml, 35% loss of activity after 1 month | Acetoanaerobium sticklandii |
| 5.4.3.5 | 4°C, 20% glycerol v/v or 0.02 mM coenzyme B12, enzyme concentration 1.0 mg/ml, 9% loss of activity after 2 d | Acetoanaerobium sticklandii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.12 | L-Ornithine | isomeric form not specified | Acetoanaerobium sticklandii | D-Ornithine | the product of this reaction is not identical with D-ornithine, the literature reference is probably incorrect | ? | |
| 5.4.3.5 | D-Orn | r | Acetoanaerobium sticklandii | L(4S)-2,4-Diaminopentanoate | - |
? | |
| 5.4.3.5 | D-Orn | enzyme of ornithine fermentation | Acetoanaerobium sticklandii | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.3.5 | dimer | 2 * 95000-98000, SDS-PAGE | Acetoanaerobium sticklandii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.5 | 37 | - |
- |
Acetoanaerobium sticklandii |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.5 | 23 | 49 | half maximal activities at 23°C and 49°C | Acetoanaerobium sticklandii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.5 | 45 | - |
rapid inactivation above 45°C | Acetoanaerobium sticklandii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.5 | 9 | - |
- |
Acetoanaerobium sticklandii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.5 | 7.4 | 9.7 | half maximal activities at pH 7.4 and 9.7 | Acetoanaerobium sticklandii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.3.5 | pyridoxal 5'-phosphate | required, Km: 0.00036 mM | Acetoanaerobium sticklandii | |
| 5.4.3.5 | vitamin B12 | required | Acetoanaerobium sticklandii | |
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