BRENDA - Enzyme Database

The zinc-binding site of Escherichia coli glutamyl-tRNA synthetase is located in the acceptor-binding domain

Liu, J.; Gagnon, Y.; Gauthier, J.; Furenlid, L.; L'Heureux, P.J.; Auger, M.; Nureki, O.; Yokoyama, S.; Lapointe, J.; J. Biol. Chem. 270, 15162-15169 (1995)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
6.1.1.17
H129Q
mutants encoding GluRS variants altered in the 98C-138C segment. Thermosensitive mutants H129Q, H131Q, H132Q and C138S. Mutants without glutamyl-tRNA synthetase activity: C100S, C125S. In the mutants C98S and H127Q the activity is 10fold lower than in cells overproducing the wild-type enzyme or the variants H129Q, H131Q, H132Q, and C138S
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Escherichia coli
-
mutants encoding GluRS variants altered in the 98C-138C segment
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.17
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
206
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
206
Escherichia coli
-
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
6.1.1.17
H129Q
mutants encoding GluRS variants altered in the 98C-138C segment. Thermosensitive mutants H129Q, H131Q, H132Q and C138S. Mutants without glutamyl-tRNA synthetase activity: C100S, C125S. In the mutants C98S and H127Q the activity is 10fold lower than in cells overproducing the wild-type enzyme or the variants H129Q, H131Q, H132Q, and C138S
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
206
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
206
Escherichia coli
-