Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Soda, K.; Osumi, T.
    Amino acid racemase (Pseudomonas striata) (1971), Methods Enzymol., 17, 629-636.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.1.10
-
 Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.10 D-cycloserine
-
 Pseudomonas putida
5.1.1.10 hydroxylamine
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.10 30
-
 D-Lys
-
 Pseudomonas putida
5.1.1.10 33
-
 L-Leu L-2-aminobutyrate Pseudomonas putida
5.1.1.10 35
-
 L-ethionine
-
 Pseudomonas putida
5.1.1.10 36
-
 L-2-aminobutyrate L-Ala Pseudomonas putida
5.1.1.10 38
-
 L-Met
-
 Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.10 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.10
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.10 708.4
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
5.1.1.10 0-5°C, 0.01 M potassium phosphate buffer, pH 7.0, 2 mM pyridoxal 5'-phosphate, 1 month, stable Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.10 D-2,4-Diaminobutyrate 10% of the activity relative to D-Lys Pseudomonas putida L-2,4-Diaminobutyrate
-
 ?
5.1.1.10 D-Arg 60% of the activity relative to D-Lys Pseudomonas putida L-Arg
-
 ?
5.1.1.10 L-2-Aminobutyrate 4.7% of the activity relative to D-Lys Pseudomonas putida D-2-Aminobutyrate
-
 ?
5.1.1.10 L-Ala 9% of the activity relative to D-Lys Pseudomonas putida D-Ala
-
 ?
5.1.1.10 L-Citrulline 16% of the activity relative to D-Lys Pseudomonas putida D-Citrulline
-
 ?
5.1.1.10 L-Ethionine 76% of the activity relative to D-Lys Pseudomonas putida D-Ethionine
-
 ?
5.1.1.10 L-His 1.8% of the activity relative to D-Lys Pseudomonas putida D-His
-
 ?
5.1.1.10 L-Leu 3% of the activity relative to D-Lys Pseudomonas putida D-Leu
-
 ?
5.1.1.10 L-Lys
-
 Pseudomonas putida D-Lys
-
 ?
5.1.1.10 L-Met 48% of the activity relative to D-Lys Pseudomonas putida D-Met
-
 ?
5.1.1.10 L-Ser 8.7% of the activity relative to D-Lys Pseudomonas putida D-Ser
-
 ?
5.1.1.10 additional information no activity with: D-Glu, L-Ile, L-Phe, L-Thr, L-Val Pseudomonas putida ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.10 7.5 8.5
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.10 pyridoxal 5'-phosphate Km: 0.2 mM Pseudomonas putida
5.1.1.10 pyridoxal 5'-phosphate holoenzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme Pseudomonas putida