BRENDA - Enzyme Database

Glutamyl-tRNA synthetase from Thermus thermophilus HB8. Molecular cloning of the gltX gene and crystallization of the overproducing protein

Nureki, O.; Suzuki, K.; Hara-Yokoyama, M.; Kohno, T.; Matsuzawa, H.; Ohta, T.; Shimizu, T.; Morikawa, K.; Miyazawa, T.; Yokoyama, S.; Eur. J. Biochem. 204, 465-472 (1992)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.1.1.17
overexpression in Escherichia coli
Thermus thermophilus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
53901
-
x * 53901, calculation from nucleotide sequence
Thermus thermophilus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Thermus thermophilus
-
; overexpressed in Escherichia coli; wild-type and mutant enzymes
-
6.1.1.17
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.17
-
Thermus thermophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
204
Thermus thermophilus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
204
Thermus thermophilus
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
204
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
AMP + diphosphate + L-glutamyl-tRNAGlu
-
204
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
Subunits
EC Number
Subunits
Commentary
Organism
6.1.1.17
?
x * 53901, calculation from nucleotide sequence
Thermus thermophilus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
overexpression in Escherichia coli
Thermus thermophilus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
53901
-
x * 53901, calculation from nucleotide sequence
Thermus thermophilus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
-
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
204
Thermus thermophilus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
204
Thermus thermophilus
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
204
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
AMP + diphosphate + L-glutamyl-tRNAGlu
-
204
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.1.1.17
?
x * 53901, calculation from nucleotide sequence
Thermus thermophilus