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Literature summary extracted from

  • Okubo, Y.; Tomioka, R.; Yokoigawa, K.; Kawai, H.
    Lability of alanine racemase from a psychrotroph (1995), J. Home Econ. Jpn., 46, 1135-1140.
No PubMed abstract available

General Stability

EC Number General Stability Organism
5.1.1.1 5 min, about 50% loss of activity after incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide Pseudomonas fluorescens
5.1.1.1 5 min, resistant to incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide Geobacillus stearothermophilus
5.1.1.1 denatured by 3.5 M urea in one transition phase Geobacillus stearothermophilus
5.1.1.1 denatured by urea in two transition phases, dissociation of pyridoxal 5'-phosphate with 4.0 M urea, unfolding with 5.5 M urea Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.1 additional information
-
 additional information Km values of L-Ala in the presence of urea at various concentrations Geobacillus stearothermophilus
5.1.1.1 additional information
-
 additional information Km values of L-Ala in the presence of urea at various concentrations Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Geobacillus stearothermophilus
-
-
-
5.1.1.1 Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 L-Ala
-
 Geobacillus stearothermophilus D-Ala
-
 ?
5.1.1.1 L-Ala
-
 Pseudomonas fluorescens D-Ala
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.1 30
-
 over 30°C, 1 h, quick loss of activity Pseudomonas fluorescens
5.1.1.1 30
-
 5 min, about 50% loss of activity after incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide Pseudomonas fluorescens
5.1.1.1 30
-
 5 min, resistant to incubation with 0.08% SDS, 1 M guanidine hydrochloride, 4 M urea, 45% ethanol or 50% dimethyl sulfoxide Geobacillus stearothermophilus
5.1.1.1 75
-
 1 h, inactivation over 75°C Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.1.1 8.3 10.5 1 h, 0°C, stable Geobacillus stearothermophilus
5.1.1.1 8.3 10.5 1 h, 0°C, stable Pseudomonas fluorescens